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Proteomic analysis of injured storage roots in cassava (Manihot esculenta Crantz) under postharvest physiological deterioration

文献类型： 外文期刊

作者： Qin, Yuling 1 ; Djabou, Astride Stephanie Mouafi 3 ; An, Feifei 2 ; Li, Kaimian 2 ; Li, Zhaogui 4 ; Yang, Long 5 ; Wan 1 ;

作者机构： 1.South China Normal Univ, Coll Life Sci, Guangdong Prov Key Lab Biotechnol Plant Dev, Guangzhou, Guangdong, Peoples R China

2.Chinese Acad Trop Agr Sci, Key Lab, Trop Crops Genet Resources Inst, Minist Agr Germplasm Resources Conservat & Utiliz, Danzhou, Peoples R China

3.Univ Yaounde I, Higher Teachers Training Coll, Dept Biol Sci, Lab Plant Physiol, Yaounde, Cameroon

4.Agr Bur Wuming Cty, Wuming, Peoples R China

5.Guizhou Prov Acad Agr Sci, Subtrop Crops Res In

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2017 年 12 卷 3 期

页码：

收录情况： SCI

摘要： Postharvest physiological deterioration (PPD) is a global challenge in the improvement of cassava value chain. However, how to reduce cassava spoilage and reveal the mechanism of injured cassava storage roots in response to PPD were poorly understood. In the present study, we investigated the activities of antioxidant enzymes of cassava injured storage roots in PPD-susceptible (SC9) and PPD-tolerant (QZ1) genotypes at the time-points from 0h to 120h, and further analyzed their proteomic changes using two-dimensional electrophoresis (2-DE) in combination with MALDI-TOF-MS/MS. Ninety-nine differentially expressed proteins were identified from SC9 and QZ1 genotypes in the pairwise comparison of 24h/0h, 48h/0h, 72h/0h and 96h/0h. Of those proteins were associated with 13 biological functions, in which carbohydrate and energy metabolism related proteins were the biggest amount differential proteins in both genotypes, followed by chaperones, DNA and RNA metabolism, and defense system. We speculated that SOD in combination with CAT activities would be the first line of defense against PPD to support PPD-tolerant cassava varieties. The four hub proteins including CPN60B, LOS2, HSC70-1 and CPN20B, produced from the network of protein-protein interaction, will be the candidate key proteins linked with PPD. This study provides a new clue to improve cassava PPD-tolerant varieties and would be helpful to much better understand the molecular mechanism of PPD of cassava injured storage roots.

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