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Optimization of biosurfactant production from Vibrio sp BSM-30 isolated in tropical waters

文献类型： 外文期刊

作者： Su, Zengjian 1 ; Li, Min 3 ; Zhang, Yuxiu 1 ;

作者机构： 1.China Univ Min & Technol Beijng, Sch Chem & Environm Engn, Beijing 100083, Peoples R China

2.Hainan Univ, Environm & Plant Protect Coll, Haikou 570228, Hainan Province, Peoples R China

3.Environm & Plant Protect Res Inst, CATAS, Haikou 571737, Hainan Province, Peoples R China

期刊名称：INTERNATIONAL CONFERENCE ON ENERGY ENGINEERING AND ENVIRONMENTAL PROTECTION (EEEP2016)

ISSN： 1755-1307

年卷期： 2017 年 52 卷

页码：

收录情况： SCI

摘要： The strain BSM-30 (Vibrio sp.), isolated from Chinese tropical waters, could be a biosurfactant producing bacteria according with results obtained by the oil spreading method. The culture conditions for biosurfactant production were tested respectively such as inoculation (2%, 6%, 10%, 14% as setting), shaking speed(120 r/min, 150 r/min, 180 r/min as setting), temperature (25 degrees C, 30 degrees C, 35 degrees C as setting), pH (7,8,9 as setting), salinity (1.5%, 2.5%, 3.0%, 4.5%, 5.5% as setting), which results showed that the best culture conditions for BS production were 10% inoculation quantity, 180 r/min, 25 degrees C, pH 8, and 3.5% salinity. The optimization of carbon sources (20g/of glucose, 20g/L of starch, 20g/L of paraffin oil 20g/L of diesel, 20g/L of oil as setting) and nitrogen sources (6g/L of NaNO3,7.1g/L of KNO3,5.6g/L of NH4NO3,9.3g/L of (NH4)(2)SO4, 4.2g/L of CO(NH2)(2) as setting) were also tested, which results showed that the best nitrogen source and carbon source were (NH4)(2)SO4 and soluble starch.

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