Construction of a High-Density Genetic Map Based on Large-Scale Marker Development in Mango Using Specific-Locus Amplified Fragment Sequencing (SLAF-seq)
文献类型： 外文期刊
作者： Luo, Chun 1 ; Shu, Bo 1 ; Yao, Quangsheng 1 ; Wu, Hongxia 1 ; Xu, Wentian 1 ; Wang, Songbiao 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Minist Agr, Key Lab Trop Fruit Biol, South Subtrop Crops Res Inst, Zhanjiang, Peoples R China
关键词： Mangifera indica L.;SLAF-seq;high-density genetic map;SNPs;linkage group
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）
ISSN： 1664-462X
年卷期： 2016 年 7 卷
页码：
收录情况： SCI
摘要： Genetic maps are particularly important and valuable tools for quantitative trait locus (QTL) mapping and marker assisted selection (MAS) of plant with desirable traits. In this study, 173 F-1 plants from a cross between Mangifera indica L. "Jin-Hwang" and M. indica L. "Irwin" and their parent plants were subjected to high throughput sequencing and specific-locus amplified fragment (SLAF) library construction. After preprocessing, 66.02 Gb of raw data containing 330.64 M reads were obtained. A total of 318,414 SLAFs were detected, of which 156,368 were polymorphic. Finally, 6594 SLAFs were organized into a linkage map consisting of 20 linkage groups (LGs). The total length of the map was 3148.28 cM and the average distance between adjacent markers was 0.48 cM. This map could be considered, to our knowledge, the first high-density genetic map of mango, and might form the basis for fine QTL mapping and MAS of mango.
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