文献类型： 外文期刊

作者： Qiu, Tianlei ¹ ; Huo, Linhe ¹ ; Guo, Yajie ¹ ; Gao, Min ¹ ; Wang, Guoliang ¹ ; Hu, Dong ² ; Li, Cheng ³ ; Wang, Zhanwu ² ; Liu, Guiming ¹ ; Wang, Xuming ¹ ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Biotechnol, Beijing Key Lab Agr Genet Resources & Biotechnol, Beijing 100097, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Hebei Fertilizer Technol Innovat Ctr, Inst Agroresources & Environm, Shijiazhuang 050051, Hebei, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Inst Qual Stand & Testing, Beijing 100097, Peoples R China

关键词： Metagenome; Antibiotic resistance genes; Resistome; Host community; Mobile genetic elements; Livestock farm

期刊名称：ENVIRONMENTAL MICROBIOME （ 影响因子：6.36； 五年影响因子：6.36 ）

ISSN：

年卷期： 2022 年 17 卷 1 期

页码：

收录情况： SCI

摘要： Background Antibiotics and antibiotic resistance genes (ARGs) used in intensive animal farming threaten human health worldwide; however, the common resistome, ARG mobility, and ARG host composition in different animal manures and mixed manure composts remain unclear. In the present study, metagenomic assembly and cross-sample mapping were used to comprehensively decipher the common resistome and its potential mobility and hosts in animal manure and composts. Results In total, 201 ARGs were shared among different animal (layer, broiler, swine, beef cow, and dairy cow) manures and accounted for 86-99% of total relative abundance of ARGs. Except for multidrug, sulfonamide, and trimethoprim resistance genes, the relative abundance of most ARGs in composts was significantly lower than that in animal manure. Procrustes analysis indicated that antibiotic residues positively correlated with ARG composition in manure but not in composts. More than 75% ARG subtypes were shared between plasmids and chromosomes in our samples. Transposases could play a pivotal role in mediating the transfer of ARGs between different phyla in animal manure and composting. Cross-sample mapping to contigs carrying ARGs showed that the hosts of common resistome in manure had preference on animal species, and the dominant genus of ARG host shifted from Enterococcus in manure to Pseudomonas in composts. The broad host range and linking with diverse mobile genetic elements (MGEs) were two key factors for ARGs, such as sul1 and aadA, which could survive during composting. The multidrug resistance genes represented the dominant ARGs in pathogenic antibiotic-resistant bacteria in manure but could be effectively controlled by composting. Conclusions Our experiments revealed the common resistome in animal manure, classified and relative quantified the ARG hosts, and assessed the mobility of ARGs. Composting can mitigate ARGs in animal manure by altering the bacterial hosts; however, persistent ARGs can escape from the removal because of diverse host range and MGEs. Our findings provide an overall background for source tracking, risk assessment, and control of livestock ARGs.