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The Screening and Living Conditions Optimization of Biosurfactant Bacteria in Tropical Waters

文献类型： 外文期刊

作者： Su, Zengjian 1 ; Li, Min 3 ; Zhang, Yuxiu 1 ;

作者机构： 1.China Univ Min & Technol Beijng, Sch Chem & Environm Engn, Beijing 100083, Peoples R China

2.Hainan Univ, Environm & Plant Protect Coll, Haikou 570228, Hainan Province, Peoples R China

3.Environm & Plant Protect Res Inst, CATAS, Haikou 571737, Hainan Province, Peoples R China

关键词： Screening;Biosurfactant bacteria;Living conditions

期刊名称：2015 4TH INTERNATIONAL CONFERENCE ON ENERGY AND ENVIRONMENTAL PROTECTION (ICEEP 2015)

ISSN：

年卷期： 2015 年

页码：

收录情况： SCI

摘要： The strain BBsm (Bacillus cereus) was proved to be a biosurfactant bacteria by oil spreading method, which was screened from the Chinese tropical waters by researcher in this paper. The living conditions of BBsm such as inoculation (2%, 6%, 10%, 14% as setting), shaking speed(120 r/min, 150 r/min, 180 r/min as setting), temperature(25 degrees C, 30 degrees C, 35 degrees C as setting), pH(7,8,9 as setting), salinity (1.5%, 2.5%, 3.0%, 4.5%, 5.5% as setting), carbon sources (20g/of glucose, 20g/L of starch, 20g/L of paraffin oil 20g/L of diesel, 20g/L of oil as setting) and nitrogen sources (6g/L of NaNO3. 6g/L of KNO3. 6g/L of NH4NO3. 6g/L of (NH4)(2)SO4. 6g/L of CO(NH2)(2) as setting) were tested respectively, which results showed that the most suitable culture conditions of Bsm are 10% inoculation quantity, PH 8, temperature 25 degrees C, the shaking speed 180 r/min, (NH4) 2SO4 as nitrogen source, soluble starch as carbon source, 3.5% salinity.

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