Positional cloning and characterization reveal the role of a miRNA precursor gene ZmLRT in the regulation of lateral root number and drought tolerance in maize
文献类型： 外文期刊
作者： Zhang, Ming 1 ; Chen, Yanhong 1 ; Xing, Hongyan 1 ; Ke, Wensheng 1 ; Shi, Yunlu 4 ; Sui, Zhipeng 1 ; Xu, Ruibin 1 ; Gao, Lulu 1 ; Guo, Ganggang 1 ; Li, Jiansheng 7 ; Xing, Jiewen 1 ; Zhang, Yirong 1 ;
作者机构： 1.China Agr Univ, State Key Lab Agrobiotechnol, Key Lab Crop Heterosis & Utilizat MOE, Key Lab Crop Genet Improvement, Beijing 100193, Peoples R China
2.Shandong Agr Univ, Agron Coll, Tai An 271018, Peoples R China
3.Chinese Acad Agr Sci, Inst Crop Sci, Key Lab Crop Germplasm Resources & Utilizat MARA, Beijing 100081, Peoples R China
4.China Agr Univ, Ctr Crop Funct Genom & Mol Breeding, Beijing 100193, Peoples R China
5.Yantai Sci & Technol Innovat Promot Ctr, Yantai 264003, Peoples R China
6.Beijing Acad Agr & Forestry Sci, Maize Res Inst, Beijing Key Lab Maize DNA Fingerprinting & Mol Bre, Beijing 100097, Peoples R China
7.China Agr Univ, Natl Maize Improvement Ctr China, Beijing 100193, Peoples R China
关键词： drought tolerance; lateral root number; maize; miRNA precursor; QTL mapping
期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：9.106； 五年影响因子：8.241 ）
ISSN： 1672-9072
年卷期：
页码：
收录情况： SCI
摘要： Lateral roots play essential roles in drought tolerance in maize (Zea mays L.). However, the genetic basis for the variation in the number of lateral roots in maize remains elusive. Here, we identified a major quantitative trait locus (QTL), qLRT5-1, controlling lateral root number using a recombinant inbred population from a cross between the maize lines Zong3 (with many lateral roots) and 87-1 (with few lateral roots). Fine-mapping and functional analysis determined that the candidate gene for qLRT5-1, ZmLRT, expresses the primary transcript for the microRNA miR166a. ZmLRT was highly expressed in root tips and lateral root primordia, and knockout and overexpression of ZmLRT increased and decreased lateral root number, respectively. Compared with 87-1, the ZmLRT gene model of Zong3 lacked the second and third exons and contained a 14 bp deletion at the junction between the first exon and intron, which altered the splicing site. In addition, ZmLRT expression was significantly lower in Zong3 than in 87-1, which might be attributed to the insertions of a transposon and over large DNA fragments in the Zong3 ZmLRT promoter region. These mutations decreased the abundance of mature miR166a in Zong3, resulting in increased lateral roots at the seedling stage. Furthermore, miR166a post-transcriptionally repressed five development-related class-III homeodomain-leucine zipper genes. Moreover, knockout of ZmLRT enhanced drought tolerance of maize seedlings. Our study furthers our understanding of the genetic basis of lateral root number variation in maize and highlights ZmLRT as a target for improving drought tolerance in maize.
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