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Transcriptome sequencing and analysis of the coconut leaf beetle, Brontispa longissima

文献类型： 外文期刊

作者： Yan, W. 1 ; Liu, L. 1 ; Li, C. X. 1 ; Huang, S. C. 1 ; Ma, Z. L. 3 ; Qin, W. Q. 1 ; Peng, Z. Q. 4 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Coconut Res Inst, Hainan, Peoples R China

2.Beijing Forestry Univ, Key Lab Silviculture & Conservat, Minist Educ, Beijing, Peoples R China

3.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Hainan, Peoples R China

4.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Hainan, Peoples R China

关键词： Brontispa longissima;Transcriptome;Assembly

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 3 期

页码：

收录情况： SCI

摘要： The coconut leaf beetle, Brontispa longissima, is a destructive pest of palm plants. Although its ecological and biological characteristics are well understood, its genetic information remains largely unknown. To advance our understanding of its molecular basis of biology and ecology, we sequenced and analyzed its whole transcriptome by using high-throughput Illumina paired-end sequencing technology. Approximately 8.08 Gb of clean reads were generated in a single run, which were assembled by using Trinity into 41,652 unigenes with an average length of 932 bp. By sequence similarity searches for known proteins, 23,077 (55.4%) unigenes were annotated by BLASTx searches against the NCBI non-redundant protein database. Of the unigenes assembled, 18,153 and 13,733 were assigned to Gene Ontology and Clusters of Orthologous Groups of proteins, respectively. In addition, 10,415 unigenes were mapped onto 247 pathways using the Kyoto Encyclopedia of Genes and Genomes Pathway database. These transcriptomic resources will facilitate gene identification and elucidate the molecular mechanisms of biological and ecological aspects underlying this palm pest, in order to design a new control strategy.

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