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Molecular cloning and expression analysis of the MaASR1 gene in banana and functional characterization under salt stress

文献类型： 外文期刊

作者： Miao, Hongxia 1 ; Wang, Yuan 1 ; Liu, Juhua 1 ; Jia, Caihong 1 ; Hu, Wei 1 ; Sun, Peiguang 2 ; Jin, Zhiqiang 3 ; Xu, Biyu 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Trop Crop Biotechnol, Minist Agr, Haikou 571101, Hainan Province, Peoples R China

2.Chinese Acad Trop Agr Sci, Key Lab Genet Improvement Bananas, Haikou Expt Stn, Haikou 570102, Hainan Province, Peoples R China

3.Ch

关键词： ASR;Banana;Expression patterns;Salt resistance

期刊名称：ELECTRONIC JOURNAL OF BIOTECHNOLOGY （ 影响因子：2.8； 五年影响因子：3.379 ）

ISSN： 0717-3458

年卷期： 2014 年 17 卷 6 期

页码：

收录情况： SCI

摘要： Background: Abscisic acid (ABA)-, stress-and ripening-induced protein (ASR) is plant-specific hydrophilic transcriptional regulators involved in sucrose stress and wounding in banana. However, it is not known whether banana ASR genes confer salt stress tolerance. The contexts of the study was to analysis the sequence characterization of banana ASR1, and identify its expression patterns and function under salt stress using quantitative real-time PCR (qPCR) and overexpression in Arabidopsis. The purpose was to evaluate the role of banana ASR1 to salt stress tolerance employed by plants. Results: A full-length cDNA isolated from banana fruit was named MaASR1, and it had a 432 bp open reading frame (ORF) encoding 143 amino acids. MaASR1 was preferential expression in roots and leaves compared to low expression in fruits, rhizomes and flowers. Under salt stress, the expression of MaASR1 quickly increased and highest expression level was detected in roots and leaves at 4 h, and then gradually decreased. These results suggested that MaASR1 expression was induced under salt stress. MaASR1 protein was localized in the nucleus and plasma membrane. MaASR1 was transformed to Arabidopsis and verified by southern and northern analysis, transgenic lines L14 and L38 integrated one and two copies of MaASR1, respectively, while overexpression in transgenic lines provided evidence for the role of MaASR1 to salt stress tolerance. Conclusions: This study demonstrated that overexpression of MaASR1 in Arabidopsis confers salt stress tolerance by reducing the expression of ABA/stress-responsive genes, but does not affect the expression of the ABA-independent pathway and biosynthesis pathway genes. (C) 2014 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B. V. All rights reserved.

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