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Comparison of Leaf Proteomes of Cassava (Manihot esculenta Crantz) Cultivar NZ199 Diploid and Autotetraploid Genotypes

文献类型： 外文期刊

作者： An, Feifei 1 ; Fan, Jie 1 ; Li, Jun 2 ; Li, Qing X. 3 ; Li, Kaimian 1 ; Zhu, Wenli 1 ; Wen, Feng 4 ; Carvalho, Luiz J. C. 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Key Lab, Minist Agr Germplasm Resources Conservat & Utiliz, Hainan, Peoples R China

2.Jiangsu Univ, Anal & Testing Ctr, Nanjing, Jiangsu, Peoples R China

3.Univ Hawaii Manoa, Dept Mol Biosciences & Bioengn, Prote Core Facil, Manoa, HI USA

4.Guangxi Subtrop Crop Res Inst, Nanning, Peoples R China

5.EMBRAPA, Genet Resources & Biotechnol, Sobral, Ceara, Brazil

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2014 年 9 卷 4 期

页码：

收录情况： SCI

摘要： Cassava polyploid breeding has drastically improved our knowledge on increasing root yield and its significant tolerance to stresses. In polyploid cassava plants, increases in DNA content highly affect cell volumes and anatomical structures. However, the mechanism of this effect is poorly understood. The purpose of the present study was to compare and validate the changes between cassava cultivar NZ199 diploid and autotetraploid at proteomic levels. The results showed that leaf proteome of cassava cultivar NZ199 diploid was clearly differentiated from its autotetraploid genotype using 2-DE combined MS technique. Sixty-five differential protein spots were seen in 2-DE image of autotetraploid genotype in comparison with that of diploid. Fifty-two proteins were identified by MALDI-TOF-MS/MS, of which 47 were up-regulated and 5 were down-regulated in autotetraploid genotype compared with diploid genotype. The classified functions of 32 up-regulated proteins were associated with photosynthesis, defense system, hydrocyanic acid (HCN) metabolism, protein biosynthesis, chaperones, amino acid metabolism and signal transduction. The remarkable variation in photosynthetic activity, HCN content and resistance to salt stress between diploid and autotetraploid genotypes is closely linked with expression levels of proteomic profiles. The analysis of protein interaction networks indicated there are direct interactions between the 15 up-regulation proteins involved in the pathways described above. This work provides an insight into understanding the protein regulation mechanism of cassava polyploid genotype, and gives a clue to improve cassava polyploidy breeding in increasing photosynthesis and resistance efficiencies.

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