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Effect of rapid freezing-thawing techniques on the sperm parameters and ultrastructure of Chinese Taihang black goat spermatozoa

文献类型： 外文期刊

作者： Shi, Liguang 1 ; Ren, Youshe 2 ; Zhou, Hanlin 1 ; Hou, Guanyu 1 ; Xun, Wenjuan 1 ; Yue, Wenbin 2 ; Zhang, Chunxiang 1 ; Y 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Trop Crop Genet Resource Res Inst, Danzhou 571737, Hainan, Peoples R China

2.Shanxi Agr Univ, Coll Anim Sci & Vet Med, Taigu 030801, Shanxi, Peoples R China

3.Coll Anim Sci & Vet Med, Taiyuan 030024, Shanxi, Peoples R China

关键词： Spermatozoa;Freeze-thawing process;Transmission electron microscopy;Computer-assisted semen analysis;Ultrastructure

期刊名称：MICRON （ 影响因子：2.251； 五年影响因子：1.969 ）

ISSN： 0968-4328

年卷期： 2014 年 57 卷

页码：

收录情况： SCI

摘要： Supercooling sperm in liquid nitrogen vapour is a feasible and economic technique for the practical production. The study aimed to reveal the negative effects of this rapid freezing and thawing processes on Taihang black goat spermatozoa and to find out the changing of spermatozoa motility and ultrastructure by using CASA and TEM. Qualified semen samples, which collected from twenty Chinese Taihang black goats using artificial vagina were pooled and investigated the kinematics parameters and ultrastructural morphology. The results showed that freezing-thawing caused a significant reduction in the spermatozoon total motility (P< 0.001), in rapid and medium cell numbers (P< 0.001) and motility parameters (VAP, VSL, VCL, ALH and BCF) (P< 0.01). Immotile spermatozoa number was increased significantly after freezing-thawing (P <0.001). In the ultrastructural analysis, the shape with a sperm nucleus characterized by ruptures, bend and deformity was observed. The plasma membranes were broken, and nucleoplasm erupted. The mitochondria in the middle piece were disturbed by partial absence or additional accumulations. Swelling, coiling, vacuolization and structural disorganization of mitochondria were also observed. In conclusion, Freezing-thawing procedure has a detrimental effect on motility, membrane integrity and mitochondria of goat spermatozoa. Transmission electron microscopy provides an intuitive observation to investigate deformity spermatozoa. (C) 2013 Elsevier Ltd. All rights reserved.

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