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CRYOPRESERVATION OF CALLERYA SPECIOSA (CHAMP.) SCHOT THROUGH DROPLET-VITRIFICATION

文献类型： 外文期刊

作者： Li, Zhiying 1 ; Li, Tengmin 2 ; Xu, Li 1 ; Panis, Bart 3 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Crop Genet Resources, Minist Agr, Key Lab South China Gene Resources & Germplasm Cr, Danzhou Sity 571737, Hainan Province, Peoples R China

2.Hainan Univ, Haikou 571700, Hainan, Peoples R China

3.Catholic Univ Louvain, B-3001 Heverlee, Belgium

关键词： axillary buds;hairy explant;shake

期刊名称：PROPAGATION OF ORNAMENTAL PLANTS （ 影响因子：0.281； 五年影响因子：0.453 ）

ISSN： 1311-9109

年卷期： 2013 年 13 卷 4 期

页码：

收录情况： SCI

摘要： Cryopreservation is a practical method for the safe preservation of plant genetic resources. Impregnation with cryoprotective agents and the use of suitable explants are key factors for a successful procedure. It is, however, often difficult to work with "hairy" explants because of their high surface tension preventing an efficient treatment with cryoprotectant. Axillary shoot segments with one bud of Callerya speciosa were successfully cryopreserved by droplet-vitrification. The results showed that 1 mm shoot segments with one axillary bud excised from in vitro shoots could be used as explants for cryopreservation. The best pretreatment was performed with loading solution (LS), applied for 240 mm at 25 degrees C on a shaker at 150 rpm. This was followed by a plant vitrification solution (PVS2) treatment at 0 degrees C for 60 mm and rapid freezing in liquid nitrogen. Especially, rotation at 150 rpm proved to be essential to obtain high regrowth rates up to 60%.

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