Determination of ACCase Sensitivity and Gene Expression in Quizalofop-Ethyl-Resistant and -Susceptible Barnyardgrass (Echinochloa crus-galli) Biotypes
文献类型： 外文期刊
作者： Huan, Zhibo 1 ; Xu, Zhi 1 ; Lv, Daizhu 1 ; Wang, Jinxin 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Anal & Test Ctr, Haikou 571101, Hainan, Peoples R China
2.Shandong Agr Univ, Plant Protect Coll, Tai An 271018, Shandong, Peoples R China
关键词： HPLC;real-time PCR
期刊名称：WEED SCIENCE （ 影响因子：2.713； 五年影响因子：2.868 ）
ISSN： 0043-1745
年卷期： 2013 年 61 卷 4 期
页码：
收录情况： SCI
摘要： Mechanisms of herbicide resistance were studied in a quizalofop ethyl-resistant barnyardgrass biotype. Acetyl-coenzyme A carboxylase (ACCase) sensitivity to quizalofop-p-ethyl was measured by high-performance liquid chromatography and the trend in ACCase gene expression over time was determined using real-time polymerase chain reaction The results showed that an insensitive ACCase was present in Geqiushan resistant plants (R), with a resistance index of 106. The basal ACCase activities in Geqiushan Rand Geqiushan susceptible plants (S) were similar, at 1.20 and 1.17 ng malonyl-CoA min(-1) mu g(-1) extract protein, respectively. Basal ACCase gene expression in Geqiushan R was similar to that in Geqiushan S. The relative expression of ACCase gene decreased after spraying quizalofop ethyl at 60 g ai ha(-1) in Geqiushan S, whereas it was almost not changed in Geqiushan R. From these results we concluded that plastid ACCase sensitivity change might be responsible for the resistance and gene overexpression does not play a role in this resistance.
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