Metabolic Engineering of the Phenylpropanoid Pathway Enhances the Antioxidant Capacity of Saussurea involucrata
文献类型： 外文期刊
作者： Qiu, Jian 1 ; Gao, Fenghua 1 ; Shen, Guoan 1 ; Li, Chonghui 1 ; Han, Xiaoyan 1 ; Zhao, Qiao 3 ; Zhao, Dexiu 1 ; Hua, Xuej 1 ;
作者机构： 1.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Key Lab Plant Resources, Beijing, Peoples R China
2.Chinese Acad Trop Agr Sci, Rubber Res Inst, Key Lab Biol & Genet Resources Rubber Tree, Danzhou, Hainan, Peoples R China
3.Samuel Roberts Noble Fdn Inc, Div Plant Biol, Ardmore, OK 73402 USA
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2013 年 8 卷 8 期
页码：
收录情况： SCI
摘要： The rare wild species of snow lotus Saussurea involucrata is a commonly used medicinal herb with great pharmacological value for human health, resulting from its uniquely high level of phenylpropanoid compound production. To gain information on the phenylpropanid biosynthetic pathway genes in this critically important medicinal plant, global transcriptome sequencing was performed. It revealed that the phenylpropanoid pathway genes were well represented in S. involucrata. In addition, we introduced two key phenylpropanoid pathway inducing transcription factors (PAP1 and Lc) into this medicinal plant. Transgenic S. involucrata co-expressing PAP1 and Lc exhibited purple pigments due to a massive accumulation of anthocyanins. The over-expression of PAP1 and Lc largely activated most of the phenylpropanoid pathway genes, and increased accumulation of several phenylpropanoid compounds significantly, including chlorogenic acid, syringin, cyanrine and rutin. Both ABTS (2,2'-azinobis-3-ethylbenzotiazo-line-6-sulfonic acid) and FRAP (ferric reducing antioxidant power) assays revealed that the antioxidant capacity of transgenic S. involucrata lines was greatly enhanced over controls. In addition to providing a deeper understanding of the molecular basis of phenylpropanoid metabolism, our results potentially enable an alternation of bioactive compound production in S. involucrata through metabolic engineering.
- 相关文献
作者其他论文 更多>>
-
Characterization of the varied output from the anthocyanin pathway in Phalaenopsis-type Dendrobium hybrids and its relationship with flower coloration
作者：Yin, Hantai;Li, Yamei;Wu, Meiqing;Yang, Xiting;Lu, Shunjiao;Liao, Yi;Yin, Junmei;Li, Chonghui;Yin, Hantai;Wang, Yachen;Wu, Meiqing;Yang, Xiting;Wang, Yachen
关键词：Floral color; Anthocyanin; Anthocyanidin; Pigmentation
-
Virus-induced gene silencing (VIGS) for functional analysis of genes involved in the regulation of anthocyanin biosynthesis in the perianth of Phalaenopsis-type Dendrobium hybrids
作者：Hou, Tianze;Huang, Mingzhong;Liao, Yi;Lu, Shunjiao;Long, Zongxing;Yin, Junmei;Li, Chonghui;Yin, Junmei;Huang, Mingzhong;Liao, Yi;Lu, Shunjiao;Long, Zongxing;Yin, Junmei;Li, Chonghui;Hou, Tianze;Hou, Tianze
关键词：Phalaenopsis -type Dendrobium; Virus induced gene silencing (VIGS); Floral color; Anthocyanin; DhMYB2; Transcriptional regulation
-
DhMYB2 and DhbHLH1 regulates anthocyanin accumulation via activation of late biosynthesis genes in Phalaenopsis-type Dendrobium
作者：Wang, Yachen;Zhu, Wenjuan;Song, Xiqiang;Yin, Hantai;Long, Zongxing;Yin, Junmei;Li, Chonghui;Yin, Hantai;Long, Zongxing;Yin, Junmei;Li, Chonghui
关键词：Phalaenopsis-type Dendrobium; floral color; anthocyanin biosynthesis; MYB transcription factor; bHLH transcription factor
-
Establishment and application of a comprehensive assessment system for cold resistance in Denphal-group Dendrobium cultivars
作者：Lu, Shunjiao;He, Jiaqi;Yi, Shuangshuang;Liao, Yi;Li, Chonghui;Yang, Suiguang;Yin, Junmei;Lu, Shunjiao;Yi, Shuangshuang;Liao, Yi;Li, Chonghui;Yang, Suiguang;Yin, Junmei;Yin, Junmei
关键词：cold resistance; comprehensive assessment; Denphal-group Dendrobium; membership function method; physiological indicators
-
Effect of thidiazuron on morphological and flowering characteristics of Dendrobium 'Sunya Sunshine' potted plants
作者：Zhang, Dongxue;Liao, Yi;Lu, Shunjiao;Li, Chonghui;Shen, Zhen;Yang, Guangsui;Yin, Junmei;Zhang, Dongxue;Liao, Yi;Lu, Shunjiao;Li, Chonghui;Shen, Zhen;Yang, Guangsui;Yin, Junmei;Liao, Yi;Lu, Shunjiao;Li, Chonghui;Shen, Zhen;Yang, Guangsui;Yin, Junmei
关键词：Orchid; dendrobium; TDZ; floral bud formation; early flowering
-
Ectopic expression of the Anthurium andraeanum (Hort.) R2R3-MYB genes AaMYB4 and AaMYB5 enhance the flower color in transgenic tobacco
作者：Li, Chonghui;Yang, Guangsui;Huang, Surong;Yin, Junmei;Qiu, Jian;Li, Chonghui;Yang, Guangsui;Huang, Surong;Yin, Junmei
关键词：Anthocyanin; Coloration; Overexpression; Transcriptional regulation
-
AaMYB3 interacts with AabHLH1 to regulate proanthocyanidin accumulation in Anthurium andraeanum (Hort.)-another strategy to modulate pigmentation
作者：Li, Chonghui;Huang, Surong;Yin, Junmei;Yang, Guangsui;Li, Chonghui;Huang, Surong;Yin, Junmei;Yang, Guangsui;Qiu, Jian
关键词：
