文献类型： 外文期刊
作者： Lu, Peng 1 ; Li, Qinfen 2 ; Liu, Hongming 1 ; Feng, Zhaozhong 1 ; Yan, Xin 1 ; Hong, Qing 1 ; Li, Shunpeng 1 ;
作者机构： 1.Nanjing Agr Univ, Key Lab Microbiol Agr Environm, Minist Agr, Coll Life Sci, Nanjing 210095, Jiangsu, Peoples R China
2.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Danzhou 571737, Peoples R China
3.Chinese Acad Sci, State Key Lab Soil & Sustainable Agr, Inst Soil Sci, Nanjing 210008, Jiangsu, Peoples R China
关键词： Cupriavidus sp DT-1;Chlorpyrifos;3,5,6-Trichloro-2-pyridinol;Degradation pathway
期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.642； 五年影响因子：9.237 ）
ISSN： 0960-8524
年卷期： 2013 年 127 卷
页码：
收录情况： SCI
摘要： A bacterial strain, Cupriavidus sp. DT-1, capable of degrading chlorpyrifos and 3,5,6-trichloro-2-pyridinol (TCP) and using these compounds as sole carbon source was isolated and characterized. Investigation of the degradation pathway showed that chlorpyrifos was first hydrolyzed to TCP, successively dechlorinated to 2-pyridinol, and then subjected to the cleavage of the pyridine ring and further degradation. The mpd gene, encoding the enzyme responsible for chlorpyrifos hydrolysis to TCP, was cloned and expressed in Escherichia coli BL21. Inoculation of chlorpyrifos-contaminated soil with strain DT-1 resulted in a degradation rate of chlorpyrifos and TCP of 100% and 94.3%, respectively as compared to a rate of 28.2% and 19.9% in uninoculated soil. This finding suggests that strain DT-1 has potential for use in bioremediation of chlorpyrifos-contaminated environments. (C) 2012 Elsevier Ltd. All rights reserved.
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