文献类型： 外文期刊

作者： Niu, J. H. ¹ ; Jian, H. ¹ ; Guo, Q. X. ¹ ; Chen, C. L. ¹ ; Wang, X. Y. ¹ ; Liu, Q. ¹ ; Guo, Y. D. ³ ;

作者机构： 1.China Agr Univ, Dept Plant Pathol, Beijing 100193, Peoples R China

2.CATAS, Trop Crops Genet Resources Inst, Danzhou 571737, Hainan, Peoples R China

3.China Agr Univ, Dept Vegetable Sci, Beijing 100193, Peoples R China

关键词： loop-mediated isothermal amplification (LAMP);Meloidogyne enterolobii;molecular diagnosis;root-knot nematode

期刊名称：PLANT PATHOLOGY （ 影响因子：2.59； 五年影响因子：2.924 ）

ISSN： 0032-0862

年卷期： 2012 年 61 卷 4 期

页码：

收录情况： SCI

摘要： A loop-mediated isothermal amplification (LAMP) assay for detection of Meloidogyne enterolobii (Me-LAMP) was developed based on the sequences of the 5S ribosomal DNA (5S rDNA) and intergenic spacer 2 (IGS2) segment. The LAMP amplification was achieved at 65 degrees C isothermal conditions within 11.5 h. Its amplicons were confirmed using gel electrophoresis, SacI enzyme analysis, lateral flow dipstick (LFD) assay, and visual inspection through SYBR Green I and calcein staining. The results demonstrated that the Me-LAMP was able to specifically detect M. enterolobii populations from different geographical origins, with a detection limit of about 10 fg M. enterolobii genomic DNA, which was 10100 times more sensitive than conventional PCR. In addition, the applicability of LAMP to field detection was confirmed following its successful performance in detecting the pest on root and soil samples. The Me-LAMP assay possessed the characteristics of simplicity, sensitivity and specificity, and is a promising and practical molecular tool for M. enterolobii diagnosis in pest quarantine and field surveys.