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Detection and Identification of 16SrII Group Phytoplasmas Infecting Stylosanthes in China

文献类型: 外文期刊

作者: Che, Haiyan 1 ; Li, Zhengnan 2 ; Zhang, Lei 3 ; Wu, Yunfeng 2 ; Luo, Daquan 1 ;

作者机构: 1.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Danzhou 571737, Hainan, Peoples R China

2.NW A&F Univ, Coll Plant Protect, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China

3.NW A&F Univ, Coll Forestry, Yangling 712100, Shaanxi, Peoples R China

关键词: Stylosanthes guianensis witches'-broom;Stylosanthes capitata yellows;16SrII group phytoplasma;China

期刊名称:JOURNAL OF PHYTOPATHOLOGY ( 影响因子:1.789; 五年影响因子:1.574 )

ISSN: 0931-1785

年卷期: 2012 年 160 卷 7-8 期

页码:

收录情况: SCI

摘要: In July 2008, Stylosanthes guianensis with witches-broom symptoms and Stylosanthes capitata with yellows symptoms were observed in Hainan Province, China. From the symptomatic S. guianensis, the phytoplasma-specific 16S rRNA gene fragment of 1.4 kb was amplified by a direct PCR, and a fragment of 16S rRNA gene of 1.2 kb was obtained from the symptomatic Stylosanthes capitata by a nested PCR. Sequencing results and NCBI BLASTn analysis showed that the two phytoplasmas belonged to group 16SrII. Polygenetic analysis and virtual RFLP classified the two phytoplasmas into subgroup 16SrII-A.

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