Extraction of Polysaccharides from Mango (Mangifera indica Linn.) Seed by Response Surface Methodology and Identification of their Structural Characteristics
文献类型: 外文期刊
作者: Chen, Yeyuan 1 ; Luo, Haiyan 1 ; Gao, Aiping 1 ; Zhu, Min 1 ;
作者机构: 1.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Danzhou 571737, Peoples R China
关键词: DPPH radical scavenging activity;Mango seed;Polysaccharide;Structural identification
期刊名称:FOOD ANALYTICAL METHODS ( 影响因子:3.366; 五年影响因子:3.07 )
ISSN: 1936-9751
年卷期: 2012 年 5 卷 4 期
页码:
收录情况: SCI
摘要: Mango is a tropical fruit with sweet taste and desirable flavor. Mango seed polysaccharides (MSP) were extracted in this work and the extraction conditions were optimized by response surface methodology. A mathematical model was built with high fitness and the optimal conditions to obtain the highest MSP yield were 9.0 ml/g of solvent/material ratio, 174 min of time and 89 A degrees C of temperature. The monosaccharides occurred in MSP I were determined to be arabinose, rhamnose, mannose, galactose, and glucose. Besides these monosaccharides, fructose was detected as the major monosaccharide in MSP II, which relative molar percentage was 60.9%. In MSP I, mannose and galactose constructed the backbone. However, their percentage sum was only 20.7% in MSP II. Arabinose, rhamnose, and mannose were detected to have only one type of glycosidic linkage, which attended in linear structure. -> 2)-Fru-(1,4 -> was the unique linkage for fructose, and it built the branched structure. A dose-dependent behavior was observed for DPPH radical scavenging activity of MSPs I and II. Amongst, MSP I showed a better antioxidant activity than MSP II.
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