文献类型： 外文期刊
作者： Zhang, Ji 1 ; Hong, Qing 1 ; Li, Qinfen 2 ; Li, Chao 1 ; Cao, Li 1 ; Sun, Ji-Quan 1 ; Yan, Xin 1 ; Li, Shun-Peng 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Life Sci, Minist Agr, Key Lab Microbiol Engn Agr Environm, Nanjing 210095, Jiangsu, Peoples R China
2.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Danzhou 571737, Hainan Province, Peoples R China
关键词： Isoproturon;Sphingobium sp YBL2;Metabolites;Biodegradation pathway
期刊名称：INTERNATIONAL BIODETERIORATION & BIODEGRADATION （ 影响因子：4.32； 五年影响因子：4.719 ）
ISSN： 0964-8305
年卷期： 2012 年 70 卷
页码：
收录情况： SCI
摘要： In our previous study, a phenylurea herbicides degrading strain Sphingobium sp. YBL2 was isolated. In the present study, the degradation pathway of IPU (Isoproturon) in strain YBL2 was investigated by intermediate metabolites identification. HPLC and/or MS/MS analysis showed that, three intermediate metabolites, MDIPU (1-(4-isopropylphenyl)-3-methylurea), DDIPU (1-(4-isopropylphenyl) urea) and 4-IA (4-lsopropylaniline) was produced by strain YBL2. All of these intermediate metabolites could also be efficiently degraded by strain YBL2. In addition, IPU-induced aniline dioxygenase activity and catechol 2,3-dioxygenase activity were detected in strain YBL2. In combination of all these results, we deduced that the metabolic pathway of IPU in strain YBL2 involves two successive N-demethylations, followed by cleavage of the urea side chain and aromatic ring. (C) 2012 Elsevier Ltd. All rights reserved.
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