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Rapid Detection of Banana Streak Virus by Loop-mediated Isothermal Amplification Assay in South China

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文献类型：外文期刊

作者： Peng, Jun ¹ ; Fan, Zaifeng ² ; Huang, Junsheng ¹ ;

作者机构： 1.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Key Lab Monitoring & Control Trop Agr & Forest In, Danzhou 571737, Hainan, Peoples R China

2.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China

3.China Agr Univ, Minist Agr, Key Lab Plant Pathol, Beijing 100193, Peoples R China

关键词： Banana streak virus;LAMP;virus detection and management;China

期刊名称：JOURNAL OF PHYTOPATHOLOGY （影响因子：1.789；五年影响因子：1.574 ）

ISSN： 0931-1785

年卷期： 2012 年 160 卷 5 期

页码：

收录情况： SCI

摘要： Banana streak virus (BSV) is a significant constraint to banana production and genetic improvement. It is necessary to develop and use BSV detection strategies that are both reliable and sensitive for the management of the virus. A loop-mediated isothermal amplification (LAMP) assay was developed and evaluated for the detection of BSV. Four primers matching a total of six sequences of the conserved ORF III polyprotein genes were synthesized for developing a specific and sensitive LAMP for DNA extracts from field-infected banana plants. LAMP assay could detect as low as 1 pg/mu l template DNA. Test results of all field samples collected from different regions of South China showed that LAMP is more sensitive than PCR. This relatively simple and sensitive technique showed excellent potential with field-collected samples and for routine screening of tissue culture materials in South China.

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