Expression of Expansin Gene (CDK-Exp3) and Its Modulation by Exogenous Gibberellic Acid During Ripening and Softening of Persimmon Fruit
文献类型: 外文期刊
作者: Zhang, Zhengke 1 ; Fu, Runshan 1 ; Huber, Donald J. 3 ; Rao, Jingping 1 ; Chang, Xiaoxiao 1 ; Hu, Meijiao 2 ; Zhang, Y 1 ;
作者机构: 1.NW A&F Univ, Coll Hort, Yangling 712100, Peoples R China
2.Chinese Acad Trop Agr Sci, Enviroment & Plant Protect Inst, Danzhou 571737, Peoples R China
3.Univ Florida, Dept Hort Sci, IFAS, Gainesville, FL 32611 USA
关键词: persimmon;expansin;gibberellic acid;GA(3);real-time PCR;softening
期刊名称:HORTSCIENCE ( 影响因子:1.455; 五年影响因子:1.617 )
ISSN: 0018-5345
年卷期: 2012 年 47 卷 3 期
页码:
收录情况: SCI
摘要: Expansins are proteins that have been reported to contribute to fruit softening. In this study, an expansin gene, CDK-Exp3, was identified from persimmon fruit, and the mRNA accumulation of CDK-Exp3 during postharvest softening was examined using real-time polymerase chain reaction (PCR). Sequence analysis showed that CDK-Exp3 contained a putative open reading frame of 765 bp encoding a polypeptide of 254 amino acid residues, which had all the characteristics of alpha-expansin. As fruit softened, the expression of CDK-Exp3 increased dramatically within the initial 8-day ripening at 20 degrees C followed by a gradual decline at the late stages of ripening. The expression of CDK-Exp3 was inhibited by gibberellic acid, and the maximum transcript abundance was delayed by 20 days compared with that of the control fruit. The results suggest that CDK-Exp3 might be closely related to softening of persimmon fruit during postharvest ripening.
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