中国热带农业科学院机构知识库

Characterization of Novel Di-, Tri-, and Tetranucleotide Microsatellite Primers Suitable for Genotyping Various Plant Pathogenic Fungi with Special Emphasis on Fusaria and Mycospherella graminicola

收藏
分享
全文链接

文献类型：外文期刊

作者： Bahkali, Ali H. ² ; Abd-Elsalam, Kamel A. ¹ ; Guo, Jian-Rong ⁵ ; Khiyami, Mohamed A. ⁴ ; Verreet, Joseph-Alexand ¹ ;

作者机构： 1.Agr Res Ctr, Plant Pathol Res Inst, Giza, Egypt

2.King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh 1145, Saudi Arabia

3.Univ Kiel, Inst Phytopathol, D-24118 Kiel, Germany

4.King Abdulaziz City Sci & Technol KACST, Riyadh 11442, Saudi Arabia

5.Chinese Acad Trop Agr Sci, Inst Environm & Plant Protect, Danzhou 71737, Hainan, Peoples R China

关键词： genotyping;tetranucleotide microsatellites;SSR;fungal plant pathogen

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （影响因子：5.923；五年影响因子：6.132 ）

ISSN： 1661-6596

年卷期： 2012 年 13 卷 3 期

页码：

收录情况： SCI

摘要： The goals of this investigation were to identify and evaluate the use of polymorphic microsatellite marker (PMM) analysis for molecular typing of seventeen plant pathogenic fungi. Primers for di-, tri-, and tetranucleotide loci were designed directly from the recently published genomic sequence of Mycospherlla graminicola and Fusarium graminearum. A total of 20 new microsatellite primers as easy-to-score markers were developed. Microsatellite primer PCR (MP-PCR) yielded highly reproducible and complex genomic fingerprints, with several bands ranging in size from 200 to 3000 bp. Of the 20 primers tested, only (TAGG) 4, (TCC) 5 and (CA)7T produced a high number of polymorphic bands from either F. graminearum or F. culmorum. (ATG)5 led to successful amplifications in M. graminicola isolates collected from Germany. Percentage of polymorphic bands among Fusarium species ranged from 9 to 100%. Cluster analysis of banding patterns of the isolates corresponded well to the established species delineations based on morphology and other methods of phylogenetic analysis. The current research demonstrates that the newly designed microsatellite primers are reliable, sensitive and technically simple tools for assaying genetic variability in plant pathogenic fungi.

相关文献

[1]Whole genome re-sequencing and transcriptome analysis of the Stylosanthes Anthracnose pathogen Colletotrichum gloeosporioides reveal its characteristics. Huang, H. P.,Huang, H. P.,Huang, J. H.,Zheng, J. L.,Yi, K. X.,Ma, S.. 2016

[2]Genetic Diversity Analysis of Pepper Inbred Lines. Liu, Ziji,Yang, Yan,Cao, Zhenmu. 2015

[3]Development of microsatellite markers in Cocos nucifera and their application in evaluating the level of genetic diversity of Cocos nucifera. Xiao, Yong,Luo, Yi,Yang, Yaodong,Fan, Haikuo,Xia, Wei,Zhao, Songlin,Qiao, Fei,Fan, Haikuo,Sager, Ross,Mason, Annaliese S.,Mason, Annaliese S.. 2013

[4]Development of gene-based simple sequence repeat markers for association analysis in Cocos nucifera. Xia, Wei,Xiao, Yong,Liu, Zheng,Luo, Yi,Fan, Haikuo,Yang, Yaodong,Zhao, Songlin,Mason, Annaliese S.,Fan, Haikuo,Peng, Ming. 2014

[5]Characterization and utilization of microsatellites in the Coffea canephora genome to assess genetic association between wild species in Kenya and cultivated coffee. Ogutu, Collins,Fang, Ting,Wang, Lu,Ma, Baiquan,Deng, Xianbao,Owiti, Albert,Han, Yuepeng,Ogutu, Collins,Owiti, Albert,Han, Yuepeng,Yan, Lin,Huang, Lifang,Wang, Xiaoyang,Ogutu, Collins,Fang, Ting,Ma, Baiquan,Owiti, Albert,Nyende, Aggrey. 2016

[6]Constructing a Genetic Linkage Map Using an F-1 Population of Non-inbred Parents in Cassava (Manihot esculenta Crantz). Chen, Xin,Xia, Zhiqiang,Fu, Yuhua,Lu, Cheng,Wang, Wenquan.

[7]Construction of Progeny Population with Resistance to Mango Anthracnose and Their Identification by SSRs. Yao, Q. S.,Ma, X. W.,Lei, X. T.,Zhan, R. L.,Wang, S. B.,Wu, H. X..

[8]CONSTRUCTION AND CHARACTERIZATION OF A TENTATIVE AMPLIFIED FRAGMENT LENGTH POLYMORPHISM-SIMPLE SEQUENCE REPEAT LINKAGE MAP OF LAMINARIA (LAMINARIALES, PHAEOPHYTA). Yang, Guanpin,Sun, Ying,Shi, Yuanyuan,Zhang, Linan,Guo, Shanshan,Li, Bingjun,Li, Xiaojie,Li, Zhiling,Cong, Yizhou,Zhao, Yushan,Wang, Wenquan.

[9]云南超高产橡胶树的SSR、AFLP和TRAP分析. 张燕燕,黄华孙,胡彦师,程汉,安泽伟. 2010

[10]热辣2号辣椒纯度鉴定及优良自交系遗传多样性分析. 刘子记,杨衍,孙继华,刘昭华,曹振木. 2014

[11]芒果SSR-PCR反应体系的优化. 黄丽芳,雷新涛,姚全胜,刘洋,苏俊波,詹儒林. 2010

[12]利用SSR标记鉴定热研二号油绿苦瓜杂交种纯度. 刘子记,詹园凤,杨衍. 2014

[13]椰子种质资源遗传多样性的SSR分析. 周丽霞,曹红星. 2018

[14]SSR和AFLP分子标记鉴定巴西橡胶树种质资源的比较研究. 钟淦彬,李维国,位明明,郑学项,易晓洁. 2016

[15]芒果实生资源遗传多样性的SSR分析. 黄丽芳,闫林,范睿,姚全胜,刘洋,雷新涛. 2011

[16]红毛丹SSR-PCR反应体系优化及多态性标记筛选. 林兴娥,牛俊海,陈莹,马蔚红,臧小平,葛宇,王甲水,周兆禧. 2018

[17]基于SSR标记构建葡萄种质资源分子身份证. 杜晶晶,刘国银,魏军亚,刘德兵,杨小振. 2013

[18]橡胶树四倍体来源鉴定及不同倍性叶片解剖结构比较. 张源源,岳秦伊,濮寿琴,郑瑶,祝青,方家林,黄肖,李维国. 2019

[19]橡胶草转录组SSR信息分析与标记开发. 甘霖,吴景,杨玉双,张继川,覃碧,王学卓,刘实忠. 2019

[20]鹅掌楸苗期生长杂种优势的SSR分析. 王晓阳,李火根. 2011

Characterization of Novel Di-, Tri-, and Tetranucleotide Microsatellite Primers Suitable for Genotyping Various Plant Pathogenic Fungi with Special Emphasis on Fusaria and Mycospherella graminicola

作者其他论文更多>>

意见箱

Characterization of Novel Di-, Tri-, and Tetranucleotide Microsatellite Primers Suitable for Genotyping Various Plant Pathogenic Fungi with Special Emphasis on Fusaria and Mycospherella graminicola

作者其他论文 更多>>

意 见 箱

作者其他论文更多>>

意见箱