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Differentially expressed genes implicated in embryo abortion of mango identified by suppression subtractive hybridization

文献类型： 外文期刊

作者： He, J. H. 1 ; Ma, F. W. 1 ; Chen, Y. Y. 2 ; Shu, H. R. 1 ;

作者机构： 1.NW A&F Univ, Coll Hort, Yangling, Peoples R China

2.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Danzhou, Peoples R China

3.Shandong Agr Univ, Coll Hort Sci & Engn, Tai An, Shandong, Peoples R China

关键词： Mango (Mangifera indica L.);Seed embryo abortion;Subtractive suppression hybridization;Expressed sequence tags

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2012 年 11 卷 4 期

页码：

收录情况： SCI

摘要： Embryo abortion in mango severely damages mango production worldwide. The mechanisms by which the mango embryos abort have long been an intriguing question. We used subtractive suppression hybridization to investigate the differentially expressed genes involved in this process. We generated 2 cDNA libraries from normal seed and aborted seed embryos of mango cultivar 'Jinhuang'. One thousand five hundred and seventy-two high-quality expressed sequence tags (ESTs) were obtained, with 1092 from the normal seed tester library and 480 from the aborted seed tester library. These ESTs were assembled into 783 unigenes, including 147 contigs and 636 singletons in contigs; 297 singletons in gene ontology (GO) indicated coverage of a broad range of GO categories. Seven candidate genes from different categories were selected for semi-quantitative PCR analysis, and their possible functions in embryo abortion are discussed. These data provide new insight into the genetic regulation of embryo abortion in mango and may aid in further identification of novel genes and their functions.

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