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Extraction and Purification of Astaxanthin from Shrimp Shell with Alkaline-organic-solvent Method

文献类型： 外文期刊

作者： Liu, Hongchao 1 ; Li, Peng 2 ; Wang, Guang 1 ; Yu, Heping 1 ; Zeng, Zongqiang 1 ; Yang, Dan 3 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Agr Prod Proc Res Inst, Zhanjiang 524001, Peoples R China

2.Affiliated Hosp Guangdong Med Coll, Zhanjiang 524001, Peoples R China

3.Guangdong Ocean Univ, Coll Sci, Zhanjiang 524088, Peoples R China

关键词： Astaxanthin;Extraction;Purification;Analysis

期刊名称：BIOTECHNOLOGY, CHEMICAL AND MATERIALS ENGINEERING, PTS 1-3

ISSN： 1022-6680

年卷期： 2012 年 393-395 卷

页码：

收录情况： SCI

摘要： The Alkaline-organic-solvent Method was employed to extract astaxanthin from shrimp shell in this work. The results demonstrated that the optimum extraction temperature was 20 degrees C, the optimum ratio of extraction agent to sample is 1:3. Centrifugal thin layer chromatography was used to purify the astaxanthin and the optimum developer was petroleum, acetone and methanol (14:5:1). The FT-IR spectra and the HPLC analysis both demonstrated the purified substance was identical with standard astaxanthin and the content of astaxanthin account for 55.4%.

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