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A SIMPLE AND EFFICIENT METHOD FOR ISOLATION OF PINEAPPLE PROTOPLASTS

文献类型: 外文期刊

作者: Zhao, Weifeng 1 ; Yang, Wenxiu 2 ; Wei, Changbin 1 ; Sun, Guangming 1 ;

作者机构: 1.CATAS, Inst S Subtrop Crops, Zhanjiang, Peoples R China

2.Yunnan Vocat Coll Trop Plant, Dept Landscape Architecture & Hort, Puer, Peoples R China

关键词: pineapple;protoplast;isolation;enzyme

期刊名称:BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT ( 影响因子:1.632; 五年影响因子:2.029 )

ISSN: 1310-2818

年卷期: 2011 年 25 卷 3 期

页码:

收录情况: SCI

摘要: Protoplasts have showed great value for new germplasms generation. A method was described for the isolation of large numbers of pineapple protoplasts. The procedure utilized 5% mannitol solution as the primary osmoticum and an enzyme mixture of 1.5% macerozyme R-10, 1.5% cellulase R-10 and 5% mannitol solution, pH 5.5, followed by differential and gradient centrifugations. Overall, the method was found to be a simplified and effective alternative to those previously described for pineapple protoplast isolation, obtaining the highest protoplast yield (3.6 x 10(6)/g FW) and the highest protoplast viability (88.9%). Biotechnol. & Biotechnol. Eq. 2011, 25(3), 2464-2467

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