您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Isolation of the endosperm-specific LPAAT gene promoter from coconut (Cocos nucifera L.) and its functional analysis in transgenic rice plants

文献类型： 外文期刊

作者： Xu, Li 2 ; Ye, Rongjian 4 ; Zheng, Yusheng 1 ; Wang, Zhekui 1 ; Zhou, Peng 3 ; Lin, Yongjun 4 ; Li, Dongdong 1 ;

作者机构： 1.Hainan Univ, Minist Educ, Key Lab Trop Biol Resources, Haikou 570228, Hainan, Peoples R China

2.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Minist Agr, Key Lab Trop Crops Germplasm Resources Utilizat, Danzhou 571737, Hainan, Peoples R China

3.Chinese Acad Trop Agr Sci, Minist Agr, Inst Trop Biosci & Biotechnol, Key Lab Trop Crop Biotechnol, Danzhou 571737, Hainan, Peoples R China

4.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

5.Huazhong Agr Univ, Natl Ctr Plant Gene Res, Wuhan 430070, Peoples R China

关键词： Coconut (Cocos nucifera L.);Endosperm-specific;Function analysis;Promoter;Transgenic rice

期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

ISSN： 0721-7714

年卷期： 2010 年 29 卷 9 期

页码：

收录情况： SCI

摘要： As one of the key tropical crops, coconut (Cocos nucifera L.) is a member of the monocotyledonous family Aracaceae (Palmaceae). In this study, we amplified the upstream region of an endosperm-specific expression gene, Lysophosphatidyl acyltransferase (LPAAT), from the coconut genomic DNA by chromosome walking. In this sequence, we found several types of promoter-related elements including TATA-box, CAAT-box and Skn1-motif. In order to further examine its function, three different 5'-deletion fragments were inserted into pBI101.3, a plant expression vector harboring the LPAAT upstream sequence, leading to pBI101.3-L1, pBI101.3-L2 and pBI101.3-L3, respectively. We obtained transgenic plants of rice by Agrobacterium-mediated callus transformation and plant regeneration and detected the expression of gus gene by histochemical staining and fluorometric determination. We found that gus gene driven by the three deletion fragments was specifically expressed in the endosperm of rice seeds, but not in the empty vector of pBI101.3 and other tissues. The highest expression level of GUS was at 15 DAF in pBI101.3-L3 and pBI101.3-L2 transgenic lines, while the same level was detected at 10 DAF in pBI101.3-L1. The expression driven by the whole fragment was up to 1.76- and 2.8-fold higher than those driven by the -817 bp and -453 bp upstream fragments, and 10.7-fold higher than that driven by the vector without the promoter. Taken together, our results strongly suggest that these promoter fragments from coconut have a significant potential in genetically improving endosperm in main crops.

  • 相关文献

[1]AtCesA8-driven OsSUS3 expression leads to largely enhanced biomass saccharification and lodging resistance by distinctively altering lignocellulose features in rice. Fan, Chunfen,Feng, Shengqiu,Huang, Jiangfeng,Wang, Yanting,Wu, Leiming,Li, Xukai,Wang, Lingqiang,Tu, Yuanyuan,Xia, Tao,Li, Jingyang,Peng, Liangcai,Fan, Chunfen,Feng, Shengqiu,Huang, Jiangfeng,Wang, Yanting,Wu, Leiming,Li, Xukai,Wang, Lingqiang,Tu, Yuanyuan,Xia, Tao,Peng, Liangcai,Fan, Chunfen,Feng, Shengqiu,Huang, Jiangfeng,Wang, Yanting,Wu, Leiming,Li, Xukai,Wang, Lingqiang,Tu, Yuanyuan,Li, Jingyang,Peng, Liangcai,Xia, Tao,Li, Jingyang,Cai, Xiwen. 2017

[2]Differential responses of lipid peroxidation and antioxidants in Alternanthera philoxeroides and Oryza sativa subjected to drought stress. Gao, Jianming,Xiao, Qiang,Ding, Liping,Chen, Mingjie,Yin, Liang,Li, Jinzhi,Zhou, Shiyi,He, Guangyuan,Gao, Jianming.

[3]Cloning and Analysis of Vacuolar Invertase Gene (MeVINV2) Promoter from Cassava (Manihot Esculenta Crantz). Liu, Jiao,Hu, Yan-ping,Xia, Wen-rui,Yao, Yuan,Zhou, Yang,Fu, Shao-ping,Duan, Rui-jun,Li, Rui-mei,Guo, Jian-chun. 2014

[4]HbMADS4, a MADS-box Transcription Factor from Hevea brasiliensis, Negatively Regulates HbSRPP. Li, Hui-Liang,Wei, Li-Ran,Guo, Dong,Wang, Ying,Zhu, Jia-Hong,Chen, Xiong-Ting,Peng, Shi-Qing. 2016

[5]An Fe Deficiency Responsive Element with a Core Sequence of TGGCA Regulates the Expression of FEA1 in Chlamydomonas reinharditii. Fei, Xiaowen,Yang, Jinghao,Deng, Xiaodong,Fei, Xiaowen,Eriksson, Mats. 2009

[6]Low-Temperature- and Phosphate Deficiency-Responsive Elements Control DGTT3 Expression in Chlamydomonas reinhardtii. Fei, Xiaowen,Li, Ping,Li, Xinghan,Deng, Xiaodong. 2018

[7]Characterization of the Dioscorin Gene Family in Dioscorea alata Reveals a Role in Tuber Development and Environmental Response. Liu, Linya,Huang, Yacheng,Wu, Wenqiang,Xu, Yun,Cong, Ziwen,Xie, Jun,Xia, Wei,Huang, Dongyi,Liu, Linya,Huang, Yacheng,Huang, Xiaolong,Yang, Jianghua. 2017

[8]Promoter regulatory domain identification of cassava starch synthase IIb gene in transgenic tobacco. Guan, Zhihui,Chen, Xin,Xie, Hairong,Wang, Wenquan,Guan, Zhihui,Chen, Xin,Xie, Hairong,Wang, Wenquan.

[9]Molecular characterization of HbCZF1, a Hevea brasiliensis CCCH-type zinc finger protein that regulates hmg1. Guo, Dong,Yi, Hong-Yan,Li, Hui-Liang,Liu, Chen,Yang, Zi-Ping,Peng, Shi-Qing,Yi, Hong-Yan,Liu, Chen,Yang, Zi-Ping.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台