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Genome shuffling of Propionibacterium shermanii for improving vitamin B12 production and comparative proteome analysis

文献类型： 外文期刊

作者： Zhang, Ying 1 ; Liu, Jian-Zhong 1 ; Huang, Jun-Sheng 3 ; Mao, Zong-Wan 1 ;

作者机构： 1.Sun Yat Sen Univ, Biotechnol Res Ctr, Guangzhou 510275, Guangdong, Peoples R China

2.Sun Yat Sen Univ, Key Lab Biocontrol, Minist Educ, Key Lab Gene Engn, Guangzhou 510275, Guangdong, Peoples R China

3.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Danzhou 571737, Peoples R China

关键词： Vitamin B12;Genome shuffling;Propionibacterium shermanii;Proteome analysis

期刊名称：JOURNAL OF BIOTECHNOLOGY （ 影响因子：3.307； 五年影响因子：3.778 ）

ISSN： 0168-1656

年卷期： 2010 年 148 卷 2-3 期

页码：

收录情况： SCI

摘要： Genome shuffling is an efficient approach for the rapid improvement of microbial phenotype. Here we improved vitamin B12 production of Propionibacterium shermanii by genome shuffling based on inactivated protoplast fusion. A genome shuffling strain with titer of vitamin B12 of 2.85 mg l(-1), named Propionibacterium shermanii-F2-3, was obtained. The genome shuffled strain produced about 61% improvement of vitamin 812 over the parent strain after 96h. Comparative analysis of proteome profile was conducted between Propionibacterium shermanii 17 and F2-3. The expression levels of 38 proteins varied significantly in the genome shuffled strain compared with those in the parent strain. Of these proteins, 22 proteins were up-regulated, 16 proteins were down-regulated. Of the up-regulated proteins, 6 proteins (glutaminyl-tRNA synthetase (GInS), Delta-aminolevulinic acid dehydratase (HemB), methionine synthase (Meth), riboflavin synthase (RibE), phosphofructo kinase (PfkA) and isocitrate dehydrogenase (Icd) is involved in the vitamin B12 biosynthesis pathway. They may be the key enzymes of vitamin B12 biosynthesis. (C) 2010 Elsevier B.V. All rights reserved.

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