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Cloning and expression analysis of rubredoxin from cold-treated banana leaves

文献类型: 外文期刊

作者: Feng, R. J. 2 ; Lu, L. F. 3 ; Yuan, K. H. 1 ; Cheng, P. 1 ; Zhang, L. L. 1 ; Qi, J. F. 1 ; Ren, Y. 4 ; Xu, X. L. 5 ; Zhang, 1 ;

作者机构: 1.Hainan Univ, Coll Agron, Haikou 570228, Peoples R China

2.CATAS, Inst Trop Biosci & Biotechnol, Minist Agr, Key Lab Trop Crop Biotechnol, Haikou 571101, Peoples R China

3.Hainan Inst Sci & Technol, Haikou 571126, Peoples R China

4.CATAS, Trop Crops Genet Resources Inst, Danzhou 571737, Peoples R China

5.CATAS, Environm & Plant Protect Inst, Danzhou 571737, Peoples R China

关键词: Rubredoxin;Expression;Banana;Stress response

期刊名称:PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY ( 影响因子:1.039; 五年影响因子:0.77 )

ISSN: 0031-9457

年卷期: 2010 年 79 卷

页码:

收录情况: SCI

摘要: A banana (Musa AAA, Cavendish subgroup cv. Brazil) cDNA encoding a putative rubredoxin-like protein (MaRd1) was obtained from total RNA isolated from cold-treated banana leaves using rapid amplification of cDNA ends (RACE) technique. MaRd1 cDNA contained 597 nucleotides encoding 198 amino acids in the open reading frame. MaRd1 protein showed 56% amino acid identity with that of Pyrococcus furiosus rubredoxin (P24297). A chloroplast transit peptide and a transmembrane region were detected at the N-terminus and the C-terminus, respectively, of the deduced amino acid sequence of MaRd1 gene. Southern blotting revealed the occurrence of at least two copies of MaRd1 in the banana genome. Real time quantitative RT-PCR analysis revealed that the expression of MaRd1 gene was mainly in leaves, pseudo-stems and immature fruits, while it was barely detectable in roots and flowers. Cold and salt stresses induced higher levels of MaRd1 transcript accumulation in leaves. This finding indicated a role of MaRd1 in the response to these abiotic stresses.

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