文献类型： 外文期刊

作者： Wang YuePing ¹ ; Chen XiongTing ² ; Qiu LiJuan ¹ ;

作者机构： 1.Chinese Acad Sci, Inst Crop Sci, Key Lab Crop Germplasm & Biotechnol MOA, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 10008, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Biotechnol, Haikou 571101, Peoples R China

关键词： soybean;trypsin inhibitor;Bowman-Birk proteinase inhibitor;Kunitz trypsin inhibitor;allelic variation

期刊名称：SCIENCE IN CHINA SERIES C-LIFE SCIENCES （ 影响因子：1.61； 五年影响因子：1.148 ）

ISSN： 1006-9305

年卷期： 2008 年 51 卷 8 期

页码：

收录情况： SCI

摘要： Trypsin inhibitors have been found in various animals, plants and microorganisms. There were two types of trypsin inhibitors in soybean including Bowman-Birk protease inhibitors (BBI) and Kunitz inhibitors (KTI). The different BBI genes from wild soybean (G. soja) and cultivated soybean (G. max) formed a multigene family. We constructed a cDNA library of cultivar 'SuiNong 14' seed at the R7 growth stage using the SMART Kit. Seventeen contigs or singletons were highly homologous to soybean protease inhibitors. Contigs of 5, 35, 8 and 9 were highly homologous to BBI family members BBI-A1, BBI-A2, BBI-C and BBI-D, respectively. Sequence analyses showed there were novel allelic variations among the 4 BBI members in SuiNong 14. Based on the comparison of soybean seed cDNA libraries from different developmental stages, it was apparent that the expression of trypsin inhibitors increased during seed development in soybean. Phylogenetic analysis of BBI gene sequences among dicotyledonous and monocotyledonous plants demonstrated that these genes shared a common progenitor.