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Poplar trees (Populus canadensis Moench) initiate vegetative storage protein accumulation during new shoot development in spring

文献类型： 外文期刊

作者： Tian, WM 1 ; Peng, SQ 2 ; Hao, BZ 2 ; Wu, JL 2 ; Hu, ZH 3 ; Cui, KM;

作者机构： 1.Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China

2.Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China; Chinese Acad Trop Agr Sci, Rubber Res Inst, Hunan Provincial Key Lab Cultivat Physiol Trop Co, Danzhou 571737, Peoples R China; Chinese Acad Trop Agr Sci, Natl Key Biotechnol Lab Trop Corp, Haikou 571101, Peoples R China

3.Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China; Chinese Acad Trop Agr Sci, Rubber Res Inst, Hunan Pro

关键词： new shoot;nitrogen storage;Populus canadensis;temperate hardwoods;vegetative storage proteins

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）

ISSN： 1672-9072

年卷期： 2005 年 47 卷 6 期

页码：

收录情况： SCI

摘要： It was generally assumed that the accumulation of vegetative storage protein (VSP) in poplar trees and/or temperate hardwoods did not occur in spring. To test this assumption, the accumulation of the 32-kDa VSP and the differential expression of a gene encoding for the protein in poplars were investigated using light and electron microscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDSPAGE) and reverse transcription-polymerase chain reaction (RT-PCR). We report, for the first time, that poplar trees initiated VSP accumulation in new shoots during the development of new shoots in spring under conditions of high temperature and long days. The amount of 32-kDa VSP increased gradually in the stem of new shoots and in two-year-old branches, but there were no detectable changes in its abundance in the bark tissues of trunks during new shoot development. Based on the presence of a 286-bp DNA fragment that is identical to the VSP gene bspA, encoding for the 32-kDa VSP in Populus deltoids Bartr. ex Marsh., the differential expression of the 32-kDa VSP gene in P. canadensis Moench was revealed by RT-PCR. The results indicated that the 32-kDa VSP gene was expressed strongly in new shoots, relative weakly in two-year-old branches and was not expressed in the trunk during new shoot development. This pattern of VSP accumulation and VSP gene space-time differential expression may be an important mechanism by which stored nitrogen compounds are used preferentially to exogenously available nitrogen and, in addition, the dynamic pattern may also have a role in the regulation of nitrogen metabolism, especially nitrogen uptake by the roots.

  • 相关文献

[1]Vegetative storage protein in Litchi chinensis, a subtropical evergreen fruit tree, possesses trypsin inhibitor activity. Tian, Wei-Min,Peng, Shi-Qing,Wang, Xu-Chu,Shi, Min-Jing,Chen, Yue-Yi,Hu, Zheng-Hai.

[2]Distribution and ultrastructure of vegetative storage proteins in Leguminosae. Tian, WM,Hu, ZH. 2004

[3]Vegetative storage proteins in the tropical tree Swietenia macrophylia: seasonal fluctuation in relation to a fundamental role in the regulation of tree growth. Tian, WM,Wu, JL,Hao, BZ,Hu, ZH. 2003

[4]Fluctuation of vegetative storage proteins in the seedlings of Swietenia macrophylla, analogous to the seasonal changes of those in the shoot of the adult tree. Han, Ya-Qin,Yan, Xing-Fu,Zhang, Hua,Hu, Zheng-Hai,Hao, Bing-Zhong,Tian, Wei-Min. 2007

[5]Vegetative Storage Proteins in Meliaceae. Tian, WM,Wu, JL,Hao, BZ,Hu, ZH.

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