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Type-II secretion pathway structural gene xpsE, xylanase- and cellulase secretion and virulence in Xanthomonas oryzae pv. oryzae

文献类型： 外文期刊

作者： Sun, QH 1 ; Hu, J 2 ; Huang, GX 2 ; Ge, C 2 ; Fang, RX 3 ; He, CZ;

作者机构： 1.Chinese Acad Sci, Inst Microbiol, Natl Key Lab Plant Genom, Beijing 100080, Peoples R China

2.Chinese Acad Sci, Inst Microbiol, Natl Key Lab Plant Genom, Beijing 100080, Peoples R China; Tsing Hua Univ, Grad Sch Shenzhen, Div Life Sci, Shenzhen 518055, Peoples R China; Chinese Acad Trop Agr Sci, Natl Key Lab Trop Crop Biotechnol, Haikou 571101, Peoples R China

3.Chinese Acad Sci, Inst Microbiol, Natl Key Lab Plant Genom, Beijing 100080, Peoples R China; Tsing Hua Univ, Grad Sch She

关键词： extracellular enzymes;pathogenicity;Xanthomonas oryzae pv. oryzae;xpsE

期刊名称：PLANT PATHOLOGY （ 影响因子：2.59； 五年影响因子：2.924 ）

ISSN： 0032-0862

年卷期： 2005 年 54 卷 1 期

页码：

收录情况： SCI

摘要： Rice bacterial blight caused by Xanthomonas oryzae pv. oryzae is one of the most important diseases in rice-growing areas worldwide. Four virulence-deficient mutants were identified from a transposon mutagenesis library of X. oryzae pv. oryzae. Sequence-analysis revealed that the transposon of the four mutants inserted at different sites in the same ORF, which is homologous to the xpsE gene encoding a component of the type-II secretion system in many bacterial pathogens. Extracellular enzymes, such as xylanase and cellulase, were not secreted to the extracellular space in the mutants. Analysis of the protein profile of the extracellular, periplasmic and intracellular fractions indicated that at least two secreted proteins accumulated in the periplasmic space in the mutants. After genetic complementation of these mutants with a functional xpsE gene, the xpsE gene could express normally and the pathogenicity of the mutants and their secretion of extracellular enzymes were restored. Western blot analysis with an anticellulase antiserum also showed that cellulase was secreted normally in the complemented strains. The results show that the type-II secretion pathway structural gene xpsE is required for xylanase and cellulase secretion and full virulence in X. oryzae pv. oryzae.

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