Efficient in vitro shoot regeneration from mature apricot (Prunus armeniaca L.) cotyledons
文献类型： 外文期刊
作者： Wang, Hong 1 ; Petri, Cesar 2 ; Burgos, Lorenzo 2 ; Alburquerque, Nuria 2 ;
作者机构： 1.Gansu Acad Agr Sci, Inst Fruit & Floriculture Res, Lanzhou 730070, Peoples R China
2.CEBAS CSIC, Dept Mejora Plantas, Grp Biotecnol Frutales, Murcia 30100, Spain
关键词： Adventitious shoot;Histological study;Organogenesis;Thidiazuron, Fruit trees;Recalcitrant species
期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）
ISSN： 0304-4238
年卷期： 2013 年 160 卷
页码：
收录情况： SCI
摘要： Apricot (Prunus armeniaca L) is widely considered as a recalcitrant species for shoot regeneration and genetic transformation. Previous reports indicated the possibility to obtain good regeneration from apricot hypocotyl slices: however, we report here an efficient and novel procedure to obtain direct shoot regeneration from the proximal zone of mature apricot cotyledons. Additionally, the system worked for all genotypes tested, which makes this protocol a very interesting and useful tool. Regeneration percentages were influenced by plant growth regulators and the initial dark incubation period, the former being critical to obtain high regeneration from stored apricot cotyledons. The highest regeneration percentages were achieved with TDZ (4 or 8 mu M) and 0.25 mu M IBA in combination with two weeks dark incubation period reaching up to 67.2%, 56.8%, 66.7%, 46.3% and 66.7% regeneration percentages for 'Canino', 'Dorada', 'Moniqui', 'Real Fino' and 'ansu Maxim' (Prunus armeniaca L var. ansu Maxim), respectively. Regeneration pattern appeared as multiple shoots per cotyledon. Histological studies showed that epidermal and subepidermal cells of the cotyledon were the cells from where direct organogenesis occurred. To the best of our knowledge, this is the first report of direct in vitro regeneration from mature apricot cotyledons. (C) 2013 Elsevier B.V. All rights reserved.
- 相关文献
作者其他论文 更多>>
-
RNA-SEQ ANALYSIS REVEALS THE MECHANISM IN RESPONSE TO COLD STRESS OF PEACH cv 'Dingjiaba'
作者：Li, Xiaolan;Wang, Hong;Li, Xiaolan;Wang, Hong;Wu, Xinquan
关键词：cold stress; peach; Prunus persica; RNA-Seq.
-
Root restriction effects of nectarines grown in a non-arable land greenhouse
作者：Wang, Hong;Wang, Chenbing;Wang, Falin;Niu, Ruxuan;Wu, Xinquan;Zhu, Wei
关键词：Photosynthetic; Prunus persica var. Nectarina Maxim; Stomatal conductance; Intercellular CO2 concentration; Autotoxins; Phenolic acids
-
The responses of photosynthetic capacity, chlorophyll fluorescence and chlorophyll content of nectarine (Prunus persica var. Nectarina Maxim) to greenhouse and field grown conditions
作者：Wang, Hong;Wang, Falin;Wang, Gang;Majourhat, Khalid
关键词：Prunus persica;photosynthesis;photoinhibition;acclimation
-
Adventitious shoot regeneration from hypocotyl slices of mature apricot (Prunus armeniaca L.) seeds: A feasible alternative for apricot genetic engineering
作者：Wang, Hong;Alburquerque, Nuria;Burgos, Lorenzo;Petri, Cesar;Wang, Hong
关键词：thidiazuron: 51707-55-2;silver thiosulfate: 23149-52-2;3-indolebutyric acid: 133-32-4;kanamycin: 8063-07-8;aminoglycoside antibiotic;paromomycin: 84420-34-8;aminoglycoside antibiotic;2;4 dichlorophenoxy-acetic acid;p35SGUSINT: plasmid;dose-response curve;adventitious shoot regeneration;hypocotyl section;dark incubation period
-
Phosphomannose-isomerase as a selectable marker for transgenic plum (Prunus domestica L.)
作者：Wang, Hong;Petri, Cesar;Burgos, Lorenzo;Alburquerque, Nuria
关键词：
-
A chemical-inducible Cre-LoxP system allows for elimination of selection marker genes in transgenic apricot
作者：Petri, Cesar;Lopez-Noguera, Sonia;Wang, Hong;Garcia-Almodovar, Carlos;Alburquerque, Nuria;Burgos, Lorenzo;Wang, Hong
关键词：Fruit trees;Genetic engineering;Marker-free;Prunus armeniaca;Site-specific recombination;beta-Estradiol
-
Production of transgenic apricot plants from hypocotyl segments of mature seeds
作者：Petri, Cesar;Wang, Hong;Burgos, Lorenzo;Alburquerque, Nuria;Sanchez-Navarro, Jesus
关键词：Prunus;Agrobacterium;Antibiotic selection;Phosphinothricin;Genetic transformation
