文献类型： 外文期刊

作者： Song, Y. N. ¹ ; Marschner, P. ² ; Li, L. ² ; Bao, X. G. ³ ; Sun, J. H.; Zhang, F. S.;

作者机构： 1.China Agr Univ, Coll Resources & Environm Sci, Key Lab Plant & Soil Interact, MOE, Beijing 100094, Peoples R China

2.China Agr Univ, Coll Resources & Environm Sci, Key Lab Plant & Soil Interact, MOE, Beijing 100094, Peoples R China; Univ Adelaide, Sch Earth & Environm Sci, Adelaide, SA 5005, Australia; Gansu Acad Agr Sci, Inst Soil & Fertilizers, Lanzhou 730000, Peoples R China; Fujian Acad Agr Sci, Inst Biotechnol, Fuzhou, Peoples R China

3.China Agr Univ, Coll Resources & Environm Sci, Key Lab Plant & Soil Interact, MOE, Beijing 100094, Peoples R China; Univ Adelaide, Sch Earth & Environm Sci, Adelaide, SA 5005, Australia; Gansu Acad Agr Sci, Inst Soil & Fertilizers, Lanzhou 730000, Peop

关键词： ammonia-oxidizing bacteria;community composition;intercropping;PCR-DGGE;rhizosphere

期刊名称：BIOLOGY AND FERTILITY OF SOILS （ 影响因子：6.432； 五年影响因子：6.332 ）

ISSN： 0178-2762

年卷期： 2007 年 44 卷 2 期

页码：

收录情况： SCI

摘要： Cereal/cereal and cereal/legume intercropping systems are popular in the north, northwest, and southwest of China and often result in yield increases compared to monocropping. Rhizosphere interactions may play a significant role in the yield increases, particularly with respect to nutrient availability. The aim of this study was to investigate the effects of intercropping on N availability and community composition of ammonia-oxidizing bacteria in the rhizosphere of wheat, maize, and faba bean at different growth stages. Denaturing gradient gel electrophoresis (DGGE) based on 16S rRNA genes was used to analyze the community composition of bacterial ammonia oxidizers belonging to beta-proteobacteria. The results showed that intercropping with faba bean significantly increased nitrate concentrations in the rhizosphere of wheat and maize at the second sampling time (20 June) compared to monocropping or intercropping between maize and wheat. Intercropping significantly affected the community composition of ammonia-oxidizing bacteria in the rhizosphere compared to monocropping, and the effects were most pronounced in the maize/faba bean and wheat/maize intercropping systems when faba bean and wheat were at anthesis and maize was in seedling stage. In wheat/faba bean intercropping, the effects of intercropping on community composition of ammonia-oxidizing bacteria were less pronounced at the seedling stage of the two species but were significant at anthesis.