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Ducks as a potential reservoir for Pasteurella multocida infection detected using a new rOmpH-based ELISA

文献类型： 外文期刊

作者： Liu, Rongchang 1 ; Chen, Cuiteng 1 ; Cheng, Longfei 1 ; Lu, Ronghui 1 ; Fu, Guanghua 1 ; Shi, Shaohua 2 ; Chen, Hongme 1 ;

作者机构： 1.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fujian Anim Dis Control Technol Ctr, Fujian Prov Key Lab Avian Dis Control & Prevent, Fuzhou 350013, Fujian, Peoples R China

2.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fujian Anim Dis Control Technol Ctr, Fujian Prov Key Lab Avian Dis Control & Prevent, Fuzhou 350013, Fujian, Peo

关键词： duck;indirect ELISA;Pasteurella multocida;reservoir;rOmpH protein

期刊名称：JOURNAL OF VETERINARY MEDICAL SCIENCE （ 2020影响因子：1.267； 五年影响因子：1.348 ）

ISSN： 0916-7250

年卷期： 2017 年 79 卷 7 期

页码：

收录情况： SCI

摘要： Pasteurella multocida is an important pathogen of numerous domestic poultry and wild animals and is associated with a variety of diseases including fowl cholera. The aim of this study was to develop an indirect enzyme-linked immunosorbent assay (ELISA) based on recombinant outer-membrane protein H (rOmpH) for detection of anti-P. multocida antibodies in serum to determine their prevalence in Chinese ducks. The P. multocida ompH gene was cloned into pET32a, and rOmpH was expressed in Escherichia coli BL21 (DE3). Western blotting revealed that purified rOmpH was recognized by duck antisera against P. multocida, and an indirect ELISA was established. During analysis of serum samples (n=115) from ducks, the rOmpH ELISA showed 95.0% specificity, 100% sensitivity and a 92.0%. coefficient (95% confidence interval 0.844-0.997) as compared with a microtiter agglutination test. Among 165 randomly selected serum samples, which were collected in 2015 and originated from six duck farms across Fujian Province, China, anti-P. multocida antibodies were detected in 22.42% of apparently healthy ducks, including 25 of 90 sheldrakes (27.8%), eight of 50 Peking ducks (16.0%) and four of 25 Muscovy ducks (16%). Overall, the data suggest that rOmpH is a suitable candidate antigen for the development of an indirect ELISA for detection of P. multocida in ducks; moreover, our results showed that ducks could serve as a potential reservoir for P. multocida infection.

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