Allele-specific marker-based assessment revealed that the rice blast resistance genes Pi2 and Pi9 have not been widely deployed in Chinese indica rice cultivars
文献类型: 外文期刊
作者: Tian, Dagang 1 ; Chen, Zaijie 2 ; Chen, Ziqiang 2 ; Zhou, Yuanchang 1 ; Wang, Zonghua 3 ; Wang, Feng 2 ; Chen, Songbia 1 ;
作者机构: 1.Fujian Agr & Forestry Univ, Coll Crop Sci, Fuzhou 350002, Peoples R China
2.Fujian Acad Agr Sci, Biotechnol Res Inst, Fuzhou 350003, Peoples R China
3.Fujian Agr & Forestry Univ, Fujian Taiwan Joint Ctr Ecol Control Crop Pests, Fuzhou 350002, Peoples R China
关键词: Rice;Blast disease;Resistance gene;Molecular marker;Pi2;Pi9
期刊名称:RICE ( 影响因子:4.783; 五年影响因子:5.23 )
ISSN: 1939-8425
年卷期: 2016 年 9 卷
页码:
收录情况: SCI
摘要: Background: The most sustainable approach to control rice blast disease is to develop durably resistant cultivars. In molecular breeding for rice blast resistance, markers developed based on polymorphisms between functional and non-functional alleles of resistance genes, can provide precise and accurate selection of resistant genotypes without the need for difficult, laborious and time-consuming phenotyping. The Pi2 and Pi9 genes confer broad-spectrum resistance against diverse blast isolates. Development of allele-specific markers for Pi2 and Pi9 would facilitate breeding of blast resistant rice by using the two blast resistance genes. Result: In this work, we developed two new markers, named Pi9-Pro and Pi2-LRR respectively, targeting the unique polymorphisms of the resistant and susceptible alleles of Pi2 and of Pi9. The InDel marker Pi9-Pro differentiates three different genotypes corresponding to the Pi2/Piz-t, Pi9 and non-Pi2/Piz-t/Pi9 alleles, and the CAPS marker Pi2-LRR differentiates the Pi2 allele from the non-Pi2 allele. Based on the two newly developed markers and two available markers Pi2SNP and Pi9SNP, the presence of Pi2 and Pi9 was assessed in a set of 434 rice accessions consisting of 377 Chinese indica cultivars/breeding materials and 57 Chinese japonica cultivars/breeding materials. Of the 434 accessions tested, while one indica restorer line Huazhan was identified harboring the Pi2 resistance allele, no other rice line was identified harboring the Pi2 or Pi9 resistance alleles. Conclusions: Allele-specific marker-based assessment revealed that Pi2 and Pi9 have not been widely incorporated into diverse Chinese indica rice cultivars. Thus, the two blast resistance genes can be new gene sources for developing blast resistant rice, especially indica rice, in China. The two newly developed markers should be highly useful for using Pi2 and Pi9 in marker-assisted selection (MAS) breeding programs.
- 相关文献
作者其他论文 更多>>
-
Comparison of volatile compounds among different types of Tieguanyin oolong tea using DHI-GC-MS
作者:Su, Desen;Zheng, Yunyun;Huang, Minmin;Chen, Ziqiang;Chen, Meizhen;Yao, Qinghua;Su, Desen;Zheng, Yunyun;Huang, Minmin;Chen, Ziqiang;Chen, Meizhen;Yao, Qinghua;Chen, Lisong
关键词:
-
Editing the RR-TZF Gene Subfamily in Rice Uncovers Potential Risks of CRISPR/Cas9 for Targeted Genetic Modification
作者:Zhou, Shufen;Lu, Xiaozhuan;Wu, Weiren;Zhou, Shufen;Tian, Dagang;Liu, Huaqing;Zhang, Di;Chen, Rui;Yang, Shaohua;Wang, Feng
关键词:CRISPR/Cas9;
RR-TZF gene subfamily; rice (Oryza sativa L.); off-target effects; inheritable mutagenicity -
Quasi-Targeted Metabonomics Reveals Metabolites Associated with Antioxidant Activity of Mesona chinensis Benth Cultivar Xiaoye
作者:Niu, Yuqing;Zheng, Meixia;Zhu, Yanming;Chen, Hong;Zhu, Yujing;Su, Hailan;Tian, Dagang
关键词:
M. chinensis Benth; Xiaoye variety; secondary metabolites; antioxidant activity; UHPLC-MS -
Protein Phosphatases MoPtc5, MoPtc1, and MoPtc2 Contribute to the Vegetative Growth, Stress Adaptation, and Virulence of Magnaporthe oryzae
作者:Biregeya, Jules;Otieno, Frankline Jagero;Mabeche, Anjago Wilfred;Felix, Abah;Aimable, Nsanzinshuti;Abubakar, Yakubu Saddeeq;Aron, Osakina;Lu, Guodong;Wang, Zonghua;Tang, Wei;Biregeya, Jules;Hong, Yonghe;Chen, Meilian;Wang, Zonghua
关键词:
Magnaporthe oryzae ; synergistic; stress tolerance; protein phosphatases; pathogenesis -
Organic management improves soil P availability via increasing inorganic P solubilization in tea plantations
作者:Chang, Yunni;Wu, Zhidan;Chen, Yuzhen;Jiang, Fuying;Wang, Feng;Chang, Yunni;Wu, Zhidan;Chen, Yuzhen;Jiang, Fuying;Wang, Feng;Chang, Yunni;Wu, Zhidan;Chen, Yuzhen;Jiang, Fuying;Wang, Feng;Penuelas, Josep;Sardans, Jordi;Penuelas, Josep;Sardans, Jordi
关键词:Phosphorus availability; Soil microbial community; Functional gene; Tea plantation
-
The OsZHD1 and OsZHD2, Two Zinc Finger Homeobox Transcription Factor, Redundantly Control Grain Size by Influencing Cell Proliferation in Rice
作者:Guo, Mingliang;She, Zeyuan;Guo, Mingliang;Tian, Dagang;Zheng, Chun;Shi, Chao;Lu, Xiaozhuan;Jiang, Shuyu;Qin, Yuan
关键词:Rice; Zinc finger-homeodomain;
OsZHD1 ;OsZHD2 ; Grain size; Cell proliferation -
bk-5214S2L, an allelic variant of bk-5, as high-quality silage maize genetic resource
作者:Li, Gang;Guo, Xinrui;Chen, Ziqiang;Du, Jiyuan;Li, Xiaohu;Zhuge, Shilin;Ren, Shuolin;Wu, Min;Ma, Haoran;Ding, Haiping
关键词:maize; brittle stalk; silage; detergent fiber; mutant



