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Illumina microRNA profiles reveal the involvement of miR397a in Citrus adaptation to long-term boron toxicity via modulating secondary cell-wall biosynthesis

文献类型： 外文期刊

作者： Huang, Jing-Hao 1 ; Qi, Yi-Ping 4 ; Wen, Shou-Xing 2 ; Guo, Peng 1 ; Chen, Xiao-Min 2 ; Chen, Li-Song 1 ;

作者机构： 1.Fujian Agr & Forestry Univ, Inst Plant Nutr Physiol & Mol Biol, Fuzhou 350002, Peoples R China

2.Fujian Acad Agr Sci, Pomol Inst, Fuzhou 350013, Peoples R China

3.Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou 350002, Peoples R China

4.Fujian Acad Med Sci, Inst Mat Med, Fuzhou 350001, Peoples R China

5.Fujian Agr & Forestry Univ, Coll Resource & Environm Sci, Fuzhou 350002, Peoples R China

6.Fujian Agr & Forestry Univ, Fujian Key Lab Plant Mol & Cell Biol, Fuzhou 350002, Peoples R China

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2016 年 6 卷

页码：

收录情况： SCI

摘要： The mechanisms underlying tolerance to B-toxicity in plants are still controversial. Our previous studies indicated that B-toxicity is mainly limited to leaves in Citrus and that alternations of cell-wall structure in vascular bundles are involved in tolerance to B-toxicity. Here, miRNAs and their expression patterns were first identified in B-treated Citrus sinensis (tolerant) and C. grandis (intolerant) leaves via high-throughput sequencing. Candidate miRNAs were then verified with molecular and anatomical approaches. The results showed that 51 miRNAs in C. grandis and 20 miRNAs in C. sinensis were differentially expressed after B-toxic treatment. MiR395a and miR397a were the most significantly up-regulated miRNAs in B-toxic C. grandis leaves, but both were down-regulated in B-toxic C. sinensis leaves. Four auxin response factor genes and two laccase (LAC) genes were confirmed through 5'-RACE to be real targets of miR160a and miR397a, respectively. Up-regulation of LAC4 resulted in secondary deposition of cell-wall polysaccharides in vessel elements of C. sinensis, whereas down-regulation of both LAC17 and LAC4, led to poorly developed vessel elements in C. grandis. Our findings demonstrated that miR397a plays a pivotal role in woody Citrus tolerance to B-toxicity by targeting LAC17 and LAC4, both of which are responsible for secondary cell-wall synthesis.

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