A double antibody sandwich enzyme-linked immunosorbent assay for detection of soft-shelled turtle iridovirus antigens
文献类型： 外文期刊
作者： Zhang, M. 1 ; Yang, J. X. 3 ; Lin, X. M. 1 ; Zhu, C. H. 3 ; He, J. Q. 2 ; Liu, H. 2 ; Lin, T. L. 3 ;
作者机构： 1.Chinese Acad Inspect & Quarantine, Inst Anim & Plant Quarantine, Beijing 100029, Peoples R China
2.Shenzhen Exit Entry Inspect & Quarantine Bur, Shenzhen 518001, Guangdong, Peoples R China
3.Fujian Acad Agr Sci, Inst Biotechnol, Fuzhou 350003, Fujian, Peoples R China
关键词： Soft-shelled turtle iridovirus;Double antibodies sandwich ELISA;Monoclonal antibody;Detection method
期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）
ISSN： 0166-0934
年卷期： 2010 年 167 卷 2 期
页码：
收录情况： SCI
摘要： A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for detection of the soft-shelled turtle indovirus (STIV) was developed using a specific monoclonal antibody (mAb) against STIV and anti-STIV rabbit serum Using DAS-ELISA. the detection limit of STIV was found to be 10(3) PFU/ml The positive rate of 15 STIV samples was 100%, while the positive rate of 100 other aquatic virus samples was 0% These data show that DAS-ELISA is highly specific and sensitive for the detection of STIV In clinical tests, 128 samples isolated from pond-reared turtles were subjected to DAS-ELISA and PCR The overall agreement between the results obtained by DAS-ELISA and PCR was 98.4%. The results indicate that the DAS-ELISA method could be used for diagnosing diseases caused by STIV. (C) 2010 Elsevier B V. All rights reserved
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