文献类型： 外文期刊

作者： Lin, YK ¹ ; Lin, TL ² ; Wang, CC ² ; Wang, XT ² ; Stieger, K ² ; Klopfleisch, R ² ; Clark, TG ² ;

作者机构： 1.Cornell Univ, NYSCVM, Coll Vet Med, Dept Microbiol & Immunol, Ithaca, NY 14853 USA

2.Cornell Univ, NYSCVM, Coll Vet Med, Dept Microbiol & Immunol, Ithaca, NY 14853 USA; Fujian Acad Agr Sci, Inst Vet Med & Anim Husb, Fujian, Peoples R China; Univ Georgia, Coll Vet Med, Dept Med Microbiol & Parasitol, Athens, GA 30602 USA; Univ Leipzig, Sch Vet Med, Leipzig, Germany

关键词： GPI-anchored protein;surface antigen gene;ciliate;Ichthyophthirius multifiliis

期刊名称：MOLECULAR AND BIOCHEMICAL PARASITOLOGY （ 影响因子：1.759； 五年影响因子：2.135 ）

ISSN： 0166-6851

年卷期： 2002 年 120 卷 1 期

页码：

收录情况： SCI

摘要： The immobilization antigens (i-antigens) of Ichthyophthirius multifiliis are potential vaccine candidates for the prevention of 'white spot' disease in freshwater fish. These antigens vary with respect to antigenicity and molecular mass, and at least five i-antigen serotypes have been identified among parasite isolates thus far. In previous studies. the gene and corresponding cDNA encoding a similar to 48 kDa i-antigen from parasite isolate G1 (serotype A). had been cloned and sequenced. We now report on the isolation of two new genes, designated IAG52A[G5] and 1,4G52B[G5]. encoding similar to 52/55 kDa i-antigens from a parasite isolate representing a different serotype. namely. D. Based on their deduced sequences, the similar to 52/55 kDa gene products have the same structural features as the 48 kDa protein including hydrophobic N- and C-termini. periodic cysteine residues with the potential for metal binding, and tandemly repetitive amino acid sequence domains that span their length. Nevertheless. the products of these genes vary in their tandem repeat copy number, and share only similar to 50% homology overall. When expressed in heterologous systems. the products Of the newly described genes react strongly with monospecific polyclonal antisera against the i-antigens of serotype D and are clearly i-antigens. It would nevertheless appear that mRNA transcripts from the two genes are present at widely different levels within parasites themselves. Analysis at the protein level using 2-D SDS-PAGE would further suggest that multiple i-antigens are expressed within the same serotype at any given time. (C) 2002 Elsevier Science B.V. All rights reserved.