文献类型： 外文期刊

作者： Zheng, WW ¹ ; Nilsson, M ² ; Bergman, B ² ; Rasmussen, U ² ;

作者机构： 1.Univ Stockholm, Dept Bot, S-10691 Stockholm, Sweden

2.Univ Stockholm, Dept Bot, S-10691 Stockholm, Sweden; Fujian Acad Agr Sci, Ctr Biotechnol, Fuzhou 350003, Fujian, Peoples R China

关键词： Azolla;cyanobacteria-symbiosis;parsimony;PCR-fingerprinting;STRR

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）

ISSN： 0040-5752

年卷期： 1999 年 99 卷 7-8 期

页码：

收录情况： SCI

摘要： Symbiotically associated cyanobacteria from 18 accessions within all known species in the genus Azolla were examined and classified by the use of polymerase chain reaction (PCR)-fingerprinting. A repetitive sequence specific for cyanobacteria, the short tandemly repeated repetitive (STRR) sequence, was used as a primer in the reaction. Cyanobacterial filaments isolated directly from the Azolla leaf cavity or contained within homogenised symbiotic Azolla tissue were used as templates. Based on the fingerprint pattern, distinct differences were demonstrated between cyanobacteria isolated from the Euazolla and Rhizosperma sections. In addition, individual fingerprints were obtained from all cyanobacteria isolated from the different Azolla species. The fingerprints were used to generate a phylogenetic tree. Three clusters were distinguished: one contained the four isolates from the section Euazolla, a second the isolate from Azolla filiculoides, and a third the three isolates from the section Rhizosperma. By the use of STRR-PCR fingerprinting, new data on the taxonomy of cyanobacteria in Azolla were obtained, which have been difficult to generate by other classification methods. PCR-finger printing may, therefore, be a valuable tool for diversity and classification studies of symbiotic cyanobateria from Azolla and, as co-evolution between the cyanobacteria and its corresponding host exists the method may also be useful for the taxonomy of Azolla.