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TCS response regulator OmpR plays a major role in stress resistance, antibiotic resistance, motility, and virulence in Edwardsiella piscicida

文献类型： 外文期刊

作者： Huo, Xiaoping 1 ; Du, Chunmei 1 ; Huang, Huiqin 2 ; Gu, Hanjie 2 ; Dong, Xiwen 1 ; Hu, Yonghua 2 ;

作者机构： 1.Jiamusi Univ, Coll Life Sci, Jiamusi 154007, Peoples R China

2.CATAS, Hainan Inst Trop Agr Resources, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China

3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China

4.Hainan Prov Key Lab Funct Components Res & Utiliz, Haikou 571101, Hainan, Peoples R China

关键词： Edwardsiella piscicida; OmpR; Adversity; Motility; Virulence; Regulation

期刊名称：AQUACULTURE （ 影响因子：5.135； 五年影响因子：5.125 ）

ISSN： 0044-8486

年卷期： 2022 年 559 卷

页码：

收录情况： SCI

摘要： Two-component regulatory systems (TCSs) are omnipresent in Gram-negative bacteria and play a major role in response to changes in environmental cues and pathogenicity. Edwardsiella piscicida is a serious pathogen of fresh and seawater aquaculture industries and has attracted increasing attention. However, extremely limited TCSs have been reported in E. piscicida. In this study, the role of response regulator OmpR which belongs to TCS EnvZ/OmpR was investigated in E. piscicida. By construction of a markerless ompR in-frame mutant strain, TX01 Delta ompR, we found that (i) in comparison to the wild type TX01, TX01 Delta ompR exhibited markedly compromised tolerance to acid stress, osmotic stress, and oxidative stress; (ii) the deletion of ompR significantly changed bacterial sensitivity to multiple antibiotics, (iii) the deficiency of ompR tremendously reduced bacterial motility, (iv) the deficiency of ompR abated bacterial colonization in host immune tissue and bacterial overall virulence. These results indicate OmpR is an important participant in E. piscicida's adversity resistance and pathogenicity. As a response regulator, OmpR was demonstrated to downregulate acid resistance system cadBA and to upregulate the porin ompC and flagellum mediator flhDC. Taken together, our results illustrate that OmpR is a vital regulator that coordinates the expressions of multiple genes during the response to an adverse environment and invasion to host.

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