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Preparation and characterization of Sipunculus nudus peptide-calcium chelate: Structural insights and osteogenic bioactivity assessment

文献类型： 外文期刊

作者： Ouyang, Qianqian 1 ; Jiang, Yuwei 1 ; Liu, Lifen 1 ; Cai, Chengfeng 1 ; Bandara, Nandika 4 ; Li, Puwang 2 ; Wu, Kefeng 1 ; Hong, Hui 3 ; Chen, Liang 1 ;

作者机构： 1.Guangdong Med Univ, Sch Ocean & Trop Med, Zhanjiang 524023, Guangdong, Peoples R China

2.Chinese Acad Trop Agr Sci, South Subtrop Crop Res Inst, Zhanjiang 524091, Guangdong, Peoples R China

3.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China

4.Univ Manitoba, Dept Food & Human Nutr Sci, Winnipeg, MB, Canada

5.Univ Manitoba, Richardson Ctr Food Technol & Res, Winnipeg, MB, Canada

关键词： Peptide-calcium chelate; Squarrosus nudus; Structural properties; Osteogenic activity

期刊名称：JOURNAL OF FUNCTIONAL FOODS （ 影响因子：4.0； 五年影响因子：4.9 ）

ISSN： 1756-4646

年卷期： 2024 年 122 卷

页码：

收录情况： SCI

摘要： In order to prepare calcium supplements for efficient absorption and utilization in the human body, this study focused on synthesizing SNP-Ca by chelating Squarrosus nudus peptide (SNP) with calcium. We characterized its structure, stability, and calcium uptake properties in Caco-2 cells and its impact on osteogenic activity in vitro. Optimal preparation conditions were determined: a peptide-calcium mass ratio of 5:1, a 30-min reaction time, a temperature of 60 degrees C, and a pH of 7.0. Under these conditions, a calcium chelating rate of 68.32 % was achieved. Calcium binds to the peptide primarily via carboxyl oxygen and amino nitrogen atoms, and the morphology of SNP-Ca was similar to porous nanoflowers. Our cellular experiments revealed that SNP-Ca significantly increases calcium uptake in Caco-2 cells, stimulating proliferation, differentiation, and mineralization in MC3T3-E1 cells. Additionally, zebrafish larvae models showed enhanced bone formation following SNP-Ca administration. SNP- Ca has the potential of a novel calcium supplement.

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