中国热带农业科学院机构知识库

Synergistic mechanism of tetracycline degradation by poor-degrading microbe Serratia marcescens cooperated with insect during environmental decomposition cycle

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文献类型：外文期刊

作者： Yang, Chongrui ¹ ; Zhao, Zhengzheng ¹ ; Sun, Chen ¹ ; Gao, Bingqi ¹ ; Wu, Yushi ¹ ; Li, Mengzhe ¹ ; Chen, Liu ¹ ; Ju, Yan ¹ ; Rehman, Kashif ur ⁵ ; Yu, Chan ⁶ ; Li, Qinfen ⁷ ; van Huis, Arnold ⁸ ; Jimenez, Nuria ⁹ ; Zheng, Longyu ¹ ; Huang, Feng ¹ ; Ren, Zhuqing ² ; Yu, Ziniu ¹ ; Huang, Yongping ⁴ ; Zhang, Jibin ¹ ; Cai, Minmin ¹ ;

作者机构： 1.Huazhong Agr Univ, Coll Life Sci & Technol, Natl Engn Res Ctr Microbial Pesticides, Natl Key Lab Agr Microbiol, Wuhan 430070, Peoples R China

2.Frontiers Sci Ctr Anim Breeding & Sustainable Prod, Wuhan 430070, Peoples R China

3.Hubei Hongshan Lab, Wuhan 430070, Peoples R China

4.Shanghai Jiao Tong Univ, Sch Environm Sci & Engn, Shanghai 200000, Peoples R China

5.German Inst Food Technol DIL EV, Prof V Klitzing Str 7, D-49610 Quakenbruck, Germany

6.Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China

7.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou 571101, Peoples R China

8.Wageningen Univ & Res, Lab Entomol, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands

9.Univ Politecn Catalunya BarcelonaTech, Vilanova i la Geltru Sch Engn EPSEVG, Dept Chem Engn, BarcelonaTech, Vilanova I La Geltru 08800, Spain

10.Islamia Univ Bahawalpur, Fac Vet & Anim Sci, Dept Microbiol, Bahawalpur 63100, Pakistan

关键词： Black soldier fly larvae; Intestinal microbes; Synergistic degradation of tetracycline; Functional genes

期刊名称：BIORESOURCE TECHNOLOGY （影响因子：9.0；五年影响因子：9.5 ）

ISSN： 0960-8524

年卷期： 2025 年 435 卷

页码：

收录情况： SCI

摘要： The antibiotic residues in the environment disrupt ecological stability, ultimately pose potential risks to human health through the spread of the food chain. The degradation of antibiotics by insects is a crucial part of the environmental decomposition cycle, especially the synergistic effect between gut microbiota and host, which is a crucial biological principle for insects to alleviate environmental pollutants. It was known that the strain Serratia marcescens BSFL-6 efficiently degrade tetracycline collaborated with its host black soldier fly larvae, but the potential biological mechanism is still unclear. S. marcescens BSFL-6 mono-associated larvae (BML) tetracycline degradation system was performed for investigating the synergistic mechanism. Multiple key functional genes responsible for tetracycline synergistic degradation were analyzed through genome and transcriptome. Through overexpression and RNAi, results revealed that tet34 and UGT2B7 in S. marcescens BSFL-6 and larvae are the principal genes involved in tetracycline synergistic metabolism. Xanthine phosphoribosyltransferase (SmXPRTase) and UDP glucuronosyltransferase (HiUGTase) encoded by tet34 and UGT2B7 respectively exhibit the tetracycline metabolism function which scarcely reported. Through comprehensive analysis of tetracycline metabolites and in vitro purification of key enzymes, it could be reasonably inferred that SmXPRTase firstly participates in the degradation of tetracycline by removing dimethylamino to produce P430. Subsequently, HiUGTase metabolises P430 into bioavailable P652 through the glucuronidation, playing a crucial role in the overall degrading process. An undisclosed mechanism in degrading tetracycline by gut microbe collaborated with host was revealed, which contributes a new insight for the remediation mechanism of antibiotic contamination in the environment by insects.

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