The isolation and identification of a novel flavivirus from diseased Chinese soft-shelled turtle (Pelodiscus sinensis)
文献类型： 外文期刊
作者： Guo, Qi 1 ; Lyu, Sunjian 1 ; Shen, Weifeng 1 ; Liu, Li 1 ; Ma, Wenjun 2 ; Zhang, Mingxing 3 ; Bu, Weishao 4 ; Lou, Bao 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Hydrobiol, Hangzhou 310021, Peoples R China
2.Zhejiang Aquat Technol Extens Stn, Hangzhou 310021, Peoples R China
3.Ming Feng Freshwater Farm, Yuyao 315100, Peoples R China
4.Yunhe Cty Qingjiang Ecol Trionyx Sinensis Breeding, Lishui 310018, Peoples R China
关键词： Pelodiscus sinensis; Flavivirus; Clinic pathologic feature; Phylogenetic tree
期刊名称：VIROLOGY （ 影响因子：2.4； 五年影响因子：2.5 ）
ISSN： 0042-6822
年卷期： 2025 年 608 卷
页码：
收录情况： SCI
摘要： In recent years, mass mortality of Pelodiscus sinensis has occurred in many Chinese turtle farms and no etiological research on this disease has been conducted to date. The main clinical manifestation of sick P. sinensis was telangiectasis induced bleeding occurred in the limbs, calipash and plastron. After dissection, we found that the blood viscosity was reduced. Typical clinical manifestations included light abdominal cavity effusion, intestine and gastric wall edema and transparency. HE staining showed distinct lesions in liver, intestine, kidney, spleen, heart and lung tissues of infected P. sinensis. TEM observation showed that the spherical virions were approximately 30 nm in diameter. Partial genome of the pathogen was obtained by Illumina sequencing and then assembled and annotated. Phylogenetic analysis of the polyprotein amino acid sequences of this pathogen and other flaviviruses showed that it was closely related to Chinese soft-shelled turtle flavivirus isolate HZ-2017. RTPCR detection of this virus in the sick P. sinensis from turtle farms showed a high infection rate. QRT-PCR analysis of viral distribution in P. sinensis tissues indicated that the kidney contains the highest amount of virus. The virus was tentatively named "Pelodiscus sinensis flavivirus" (PSFV). In this study, the results of PSFV pathological characteristic and genome information laid a good foundation for further study of this virus.
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