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Hemin-graphene oxide-gold nanoflower-assisted enhanced electrochemiluminescence immunosensor for determination of prostate-specific antigen

文献类型： 外文期刊

作者： Liu, Gengjun 1 ; Guan, Xiaohan 2 ; Li, Binxiao 3 ; Zhou, Hong 1 ; Kong, Na 5 ; Wang, Haiyan 1 ;

作者机构： 1.Qingdao Univ, Dept Blood Transfus, Affiliated Hosp, Qingdao 266042, Peoples R China

2.Qingdao Univ, Med Coll, Clin Med Dept, Qingdao 266071, Peoples R China

3.Fudan Univ, Shanghai Stomatol Hosp, Engn Polymers & Inst Biomed Sci, Dept Chem,State Key Lab Mol, Shanghai 200433, Peoples R China

4.Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Key Lab Opt Elect Sensing & Analyt Chem Life Sci, Minist Educ, Qingdao 266042, Peoples R China

5.Chinese Acad Trop Agr Sci, Agr Prod Proc Res Inst, Zhanjiang 524001, Peoples R China

关键词： Electrochemiluminescence (ECL) biosensor; Hemin-graphene oxide-gold nanoflowers; Prostate-specific antigen; Peroxidase mimetics; Luminol

期刊名称：MICROCHIMICA ACTA （ 影响因子：6.408； 五年影响因子：5.888 ）

ISSN： 0026-3672

年卷期： 2022 年 189 卷 8 期

页码：

收录情况： SCI

摘要： An ultrasensitive luminol electrochemiluminescence (ECL) immunosensor was constructed for the detection of prostate specific antigen (PSA) using glucose oxidase-decorated hemin-graphene oxide-gold nanoflowers ternary nanocomposites as probes. Graphene oxide was first modified with hemin and then with gold nanoflowers through an in situ growth method, which has significantly boosted the catalytic activity of this graphene oxide-based peroxidase mimetics. The biocatalytical activity of this ECL immunosensor was thoroughly investigated to achieve selective recognition of the analyte molecules (PSA) by specific binding between antigens and antibodies. The limit of detection was calculated to be 0.32 pg mL(-1) with a signal-to-noise ratio of 3. A broad linear range from 7.5 x 10(-4) to 2.5 ng mL(-1) was obtained. Such step-by-step assembled biosensor showed controlled nanostructure and exhibited promising application in analysis of human serum samples with a recovery range of 90.6-111.8% and a RSD range of 3.9-5.5%.

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