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Detoxification enzyme is involved in the temperature effect on the toxicity of tetrachlorantraniliprole to Plutella xylostella

文献类型： 外文期刊

作者： An, Jingjie 1 ; Dou, Ya 'nan 2 ; Dang, Zhihong 1 ; Guo, Jianglong 1 ; Gao, Zhanlin 1 ; Li, Yaofa 1 ;

作者机构： 1.Hebei Acad Agr & Forestry Sci, Plant Protect Inst, Key Lab Integrated Pest Management Crops Northern, IPM Ctr Hebei Prov,Minist Agr & Rural Affairs, Baoding 071000, Peoples R China

2.Agr Specialty Ind Dev Ctr Baoding, Baoding 071000, Peoples R China

关键词： Temperature effect; Insecticides; Plutella xylostella; Tetrachlorantraniliprole; Transcriptome analysis; Detoxification enzymes

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 0048-3575

年卷期： 2023 年 195 卷

页码：

收录情况： SCI

摘要： The efficacy of insecticides is usually influenced by temperature. Insecticides can be divided into "positive", "negative" and "non-effect" temperature coefficient insecticides (TCI). To assess the temperature-dependent effect of tetrachlorantraniliprole (TET) on Plutella xylostella Linnaeus and to elucidate the mechanism of temperature affects TET toxicity, we determined the toxicity of TET against P. xylostella from 15 & DEG;C to 35 & DEG;C by leaf dipping method. Moreover, we compared the transcriptome data of the third-instar larvae treated by TET, chlorfenapyr (CHL, non-effect TCI), and the control group at 15, 25, 35 & DEG;C, respectively. The results showed that the toxicity of TET against P. xylostella increased with increasing temperature from 15 & DEG;C to 35 & DEG;C. A total of 21 differential expressed genes (DEGs) of detoxification enzymes were screened by RNA-seq, in which 10 upregulated genes (3 UGTs, 2 GSTs, 5 P450s) may involve the positive temperature effect of TET, and their expression patterns were consistent with qPCR results. Furthermore, the enzyme activities of GSTs and UGTs significantly increased after TET was treated at 15 & DEG;C. Especially, the temperature coefficient (TC) of TET was significantly reduced mixed with UGTs enzyme inhibitor 5-NI. Overall, TET showed higher insecticidal activity with increasing temperature, in which detoxifying enzymes associated with regulation of the positive temperature effect of TET on P. xylostella, such as UGTs, GSTs and P450s, are strongly involved. The transcriptome data provide in-depth information to understand the TET mechanism against diamondback moth. Most importantly, we identified detoxification enzymes that might be involved in regulating TET's positive temperature effect process, and contributed to efficient pest management.

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