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Development of a molecular gut-content identification system to identify aphids preyed upon by the natural enemy Coccinella septempunctata

文献类型： 外文期刊

作者： Xiao, Da 1 ; Xu, Qingxuan 1 ; Chen, Xu 1 ; Du, Xiaoyan 1 ; Desneux, Nicolas 2 ; Thomine, Eva 2 ; Dai, Hui-Jie 3 ; Harwood 1 ;

作者机构： 1.Beijing Acad Agr & Forest Sci, Inst Plant & Environm Protect, Beijing, Peoples R China

2.Univ Cote dAzur, INRAE, CNRS, UMR ISA, F-06000 Nice, France

3.Weifang Univ Sci & Technol, Facil Hort Lab Univ Shandong, Weifang 262700, Peoples R China

关键词： predator-prey relationship; molecular detection; gut analysis; food web; lady beetle

期刊名称：ENTOMOLOGIA GENERALIS （ 影响因子：5.625； 五年影响因子：4.207 ）

ISSN： 0171-8177

年卷期： 2021 年 41 卷 6 期

页码：

收录情况： SCI

摘要： Molecular gut-content analysis has revolutionized the study of predator-prey interactions and allowed the accurate description of food webs in the field. In this study, we identified and characterized cytochrome c oxidase subunit 1 (CO1) from the aphid Megoura japonica (Homoptera: Aphididae) and characterized its utility for molecular gut-content research. Coccinella septempunctata (Coleoptera: Coccinellidae) were selected as a representative predator of two insects, M. japonica and Aphis craccivora (Homoptera: Aphididae), for analysis of predator-prey interactions using prey-specific PCR and reverse-transcription quantitative PCR (RT-qPCR). The results of prey-specific PCR showed that with increasing M. japonica consumption, the MjCO1 PCR band was brightened and subsequently decreased with extended digestion time. RT-qPCR using Taqman-MGB fluorescence probe was also utilized to quantify predator-prey interaction between C. septempunctata and M. japonica. The MjCO1 copy number in C. septempunctata increased with greater consumption of M. japonica. Moreover, MjCO1 copy number in C. septempunctata decreased with increasing digestion time. Furthermore, analysis of CO1 copy numbers in the gut of C. septempunctata showed no feeding preference between M. japonica and A. craccivora. Our results demonstrate the utility of molecular gut-content analysis in understanding trophic relationships between these ladybirds and aphids. In addition, RT-qPCR is shown to be an effective and accurate method that should be considered for wider application in predation studies in field settings.

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