Atractylodes macrocephala Koidz. and Cuscuta chinensis Lam. extract relieves insulin resistance via PI3K/Akt signalling in diabetic Drosophila
文献类型： 外文期刊
作者： Li, Yinghong 1 ; Xu, Ye 1 ; Zhang, Biwei 1 ; Wang, Zhigang 1 ; Ma, Leilei 1 ; Sun, Longyu 1 ; Wang, Xiuping 3 ; Lin, Yimin 4 ; Li, Ji-an 1 ; Wu, Chenxi 1 ;
作者机构： 1.North China Univ Sci & Technol, Coll Tradit Chinese Med, Hebei Key Lab Integrated Tradit Chinese & Western, 21 Bohai Rd, Tangshan 063210, Peoples R China
2.North China Univ Sci & Technol, Sch Publ Hlth, 21 Bohai Rd, Tangshan 063210, Peoples R China
3.Hebei Acad Agr & Forestry Sci, Inst Coastal Agr, Tangshan 063299, Peoples R China
4.First Hosp Qinhuangdao, 258 Wenhua Rd, Qinguangdao 066000, Peoples R China
关键词： Atractylodes macrocephala Koidz; Cuscuta chinensis Lam; Insulin Resistance; Drosophila; PI3K/Akt
期刊名称：JOURNAL OF TRADITIONAL AND COMPLEMENTARY MEDICINE （ 影响因子：3.3； ）
ISSN： 2225-4110
年卷期： 2024 年 14 卷 4 期
页码：
收录情况： SCI
摘要： Background and aim: Type -2 diabetes mellitus (T2DM) is mainly characterized by insulin resistance (IR) induced by hyperglycaemia and insufficient insulin secretion. We employed a diabetic fly model to examine the effect and molecular mechanism of Atractylodes macrocephala Koidz. and Cuscuta chinensis Lam. (AMK -CCL) extract as traditional Chinese medicine in treating IR and T2DM. Experimental procedure: The contents of the active ingredients (rhamnose, xylose, mannose, and hyperoside) in AMK -CCL extract were determined by high-performance liquid chromatography. Wild -type ( Cg- GAL4/ +) or diabetic ( Cg > InR K1409A ) Drosophila flies were divided into the control group or metformin group and AMK -CCL (0.0125, 0.025, 0.05, 0.1 g/ml) groups. Food intake, haemolymph glucose and trehalose, protein, weight, triglycerides (TAG), and glycogen were measured to assess glycolipid metabolism. Phosphatidylinositol-3-kinase (PI3K)/Akt signalling was detected using fluorescent reporters [tGPH, Drosophila forkhead box O (dFoxO) - green fluorescent protein (GFP), Glut1 -GFP, 2-NBDG] in vivo . Glut1/ 3 mRNA levels and Akt phosphorylation levels were detected by quantitative polymerase chain reaction and western blotting, respectively, in vitro . Results: AMK -CCL extract contained 0.038 % rhamnose, 0.017 % xylose, 0.69 % mannose, and 0.039 % hyperoside. AMK -CCL at 0.0125 g/mL significantly suppressed the increase in circulating glucose, and the decrease in body weight, TAG, and glycogen contents of diabetic flies. AMK -CCL improved PI3K activity, Akt phosphorylation, Glut1/3 expression, and glucose uptake in diabetic flies, and also rescued diabetes -induced dFoxO nuclear localisation. Conclusions: These findings indicate that AMK -CCL extract ameliorates IR-induced diabetes via the PI3K/Akt signalling pathway, providing an experimental basis for clinical treatment.
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