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Identification of the GDP-L-Galactose Phosphorylase Gene as a Candidate for the Regulation of Ascorbic Acid Content in Fruits of Capsicum annuum L.

文献类型： 外文期刊

作者： Wang, Yixin 1 ; Wang, Zheng 2 ; Geng, Sansheng 2 ; Du, Heshan 2 ; Chen, Bin 2 ; Sun, Liang 1 ; Wang, Guoyun 2 ; Sha, Meihong 2 ; Dong, Tingting 2 ; Zhang, Xiaofen 2 ; Wang, Qian 1 ;

作者机构： 1.China Agr Univ, Coll Hort, Dept Vegetable Sci, Beijing 100193, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Beijing Vegetable Res Ctr, Beijing Key Lab Vegetable Germplasms Improvement,K, Beijing 100097, Peoples R China

关键词： Capsicum; ascorbic acid; transcriptome; WGCNA; GGP; VIGS; CCS

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN：

年卷期： 2023 年 24 卷 8 期

页码：

收录情况： SCI

摘要： Ascorbic acid (AsA) is an antioxidant with significant functions in both plants and animals. Despite its importance, there has been limited research on the molecular basis of AsA production in the fruits of Capsicum annuum L. In this study, we used Illumina transcriptome sequencing (RNA-seq) technology to explore the candidate genes involved in AsA biosynthesis in Capsicum annuum L. A total of 8272 differentially expressed genes (DEGs) were identified by the comparative transcriptome analysis. Weighted gene co-expression network analysis identified two co-expressed modules related to the AsA content (purple and light-cyan modules), and eight interested DEGs related to AsA biosynthesis were selected according to gene annotations in the purple and light-cyan modules. Moreover, we found that the gene GDP-L-galactose phosphorylase (GGP) was related to AsA content, and silencing GGP led to a reduction in the AsA content in fruit. These results demonstrated that GGP is an important gene controlling AsA biosynthesis in the fruit of Capsicum annuum L. In addition, we developed capsanthin/capsorubin synthase as the reporter gene for visual analysis of gene function in mature fruit, enabling us to accurately select silenced tissues and analyze the results of silencing. The findings of this study provide the theoretical basis for future research to elucidate AsA biosynthesis in Capsicum annuum L.

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