文献类型： 会议论文

第一作者： Min Wang

作者： Min Wang ¹ ; Xiaohong Sun ¹ ; Tianlei Qiu ¹ ; Meilin Han ¹ ; Xuming Wang ¹ ;

作者机构： 1.Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences

关键词： Harpin;Fermentation;Recombinant E. coli;Toxicity determination

会议名称： International Conference on Environmental Biotechnology and Materials Engineering

主办单位：

页码： 969-972

摘要： Harpin protein was produced by a recombinant E. coli BL21(DE3)/pET30a(+)hrpNEcc in a 1-ton fermentor, and acute oral toxicity test in rats and guinea pig skin sensitization test for Harpin protein were carried out in order to determine its toxicity. The experimental results show that the protein production is up to 4.78 g/L after 16-18 h of fermentation, and the production cost of this protein is 1.75 yuan/g. Harpin protein produced by the recombinant E. coli strain is non-toxic (LD_(50)>5000mg/kg) and has a low allergenicity (rate of sensitization is 0%). The results obtained in this study lay the foundation of the industrial manufacturing and the application on agriculture for Harpin protein.

分类号： R318.08-53