文献类型： 会议论文

第一作者： Shaoming Zhuo

作者： Shaoming Zhuo ¹ ; Jia Xie ² ; Chunyuan Wu ² ; Guangyi Li ² ;

作者机构： 1.Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences

2.College of Environment and Plant Protection of Hainan University

关键词： Eisenia Foetida;Earthworm;Coelomic Fluid;Extraction Method

会议名称： International Conference on Energy, Environment and Sustainable Development

主办单位：

页码： 3516-3519

摘要： This work explored a new water bath stimulating method to extract coelomic fluid from active Eisenia foetida. Coelomic fluid discharge amount and the coelomic fluid activity were tested to obtain the optimum extraction temperature (ET) and time. The maximum amount of coelomic fluid was obtained at 42℃, at which vermis is exact activity, while the vermis activity is inhibited at 44℃and even the vermis comprehensive death is observed at 46℃, therefore the optimum ET is 42℃; the optimum extraction time is 7 a.m. and 6 p.m. for which the volume of coelomic fluid is the most. When extracted from coelomic fluid at 7 a.m. and 11 a.m., 60 times and 10 times diluted solution obtained the highest generation rate; when it is at 3 p.m., 10 and 100 dilution times is the best; when it is at 1 p.m., 30 and 200 dilution times is the best, and 30-80 dilution times is the optimal when it is from 3 p.m..

分类号： x1-532