Purification and Characterization a Novel Alkaline Serine Protease from the Medicinal Mushroom Cordyceps sobolifera
文献类型： 会议论文
第一作者： Shou-Xian Wang
作者： Shou-Xian Wang 1 ; Guo-Qing Zhang 2 ; Shuang Zhao 3 ; Feng Xu 3 ; Lan-Qing Wang 3 ; Xiao-Li Geng 3 ; Li-Li Meng 3 ; Yu Liu 3 ; He-Xiang Wang 4 ;
作者机构： 1.Institute of Plant Protection and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China
2.Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, Beijing University of Agriculture, Beijing 102206, China
3.Institute of Plant Protection and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
4.State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China
关键词： Alkaline Serine Protease;Fruiting Bodies;Mushroom;Cordyceps sobolifera
会议名称： Congress of the International Society for Mushroom Science
主办单位：
页码： 611-618
摘要： A novel protease was purified from dried fruiting bodies of the mushroom Cordyceps sobolifera. The isolation procedure utilized ion exchange chromatography on DEAE-cellulose and SP-sepharose followed by gel filtration on Superdex 75. The protease did not adsorb on DEAE-cellulose but bound to SP-Sepharose. In sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE), the protease resolved as a single band with an apparent molecular mass of 31 kDa. It exhibited optimal activity at a temperature of 60 ℃, a pH of 12.4 with a Km of 1. 98 mg/mL and a Vmax of 23. 56 μg/mL/min against its substrate casein. Protease activity was enhanced by the Fe2+ion at low concentrations in 1. 25-10 mM and was strongly inhibited by Hg2+up to 1. 25 mM. The protease was strongly inhibited by phenylmethylsulfonyl fluoride (PMSF), suggesting that it was a serine protease. It manifested significant inhibitory activity toward HIV-1 reverse transcriptase (RT) with an IC50 value of 8. 2×10-3 μM, which was the highest anti-HIV-1 RT activity of reported mushroom proteins.
分类号： s646
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